Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김용희 | - |
dc.contributor.author | 천연지 | - |
dc.date.accessioned | 2018-04-18T06:07:01Z | - |
dc.date.available | 2018-04-18T06:07:01Z | - |
dc.date.issued | 2018-02 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/68175 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000431979 | en_US |
dc.description.abstract | It is a study on development of bio-inspired poly (lactic-co-glycolic acid) (PLGA) microparticles. Although PLGA have proved several advances as a biomaterial, a few drawbacks are still present. For therapeutic effect, the PLGA particles should cross an endothelial layer as drug carriers. I got inspired from CD14+ monocytes, inflammation related human monocytes, that migrates the endothelial layer under inflamed condition. I try to mimic the endothelial layer crossing ability by cloaking the CD14+ monocytes membranes on PLGA microparticles. Preparation of the PLGA microparticles was conducted by emulsion method using dichloromethane. CD14+ Monocytes membranes are cloaked to PLGA microparticles by sonication after going through several freeze-thaw cycles. Uniform size distribution and surface morphology was also confirmed by Transmission Electron Microscope and Scanning Electron Microscope. As the CD14 is a marker for the human monocyte, the PLGA microparticles with CD14+ monocytes membranes were visualized by confocal microscope using FITC-labeled anti-CD14 Antibody. Compared to uncoated PLGA microparticles, the PLGA microparticles with CD14+ monocytes membranes showed increased cellular uptake. Also enhanced endothelial cell layer permeability of PLGA microparticles with CD14+ monocytes membranes was observed through transwell study. In addition, substantiation of experimental result using human monocytes proposes the “node” to clinical trial for inflammatory disease. | - |
dc.publisher | 한양대학교 | - |
dc.title | 생체모방형 마이크로입자 개발 | - |
dc.title.alternative | Development of bio-inspired poly(lactic-co-glycolic acid) microparticles | - |
dc.type | Theses | - |
dc.contributor.googleauthor | 천연지 | - |
dc.sector.campus | S | - |
dc.sector.daehak | 대학원 | - |
dc.sector.department | 생명공학과 | - |
dc.description.degree | Master | - |
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