Transitional States of Acrosomal Exocytosis and Proteolytic Processing of the Acrosomal Matrix in Guinea Pig Sperm

Abstract

In this study, we adapted a FluoSphere bead‐binding assay to study the exposure and release of guinea pig sperm acrosomal components during the course of capacitation and acrosomal exocytosis. Prior to capacitation or the initiation of exocytosis, acrosomal proteins were not accessible to FluoSpheres coated with antibodies against two acrosomal matrix (AM) proteins, AM67 and AM50; during the course of capacitation and ionophore‐induced acrosomal exocytosis, however, we detected the transient exposure of the solid‐phase AM proteins on the surface of guinea pig sperm using the antibody‐coated fluorescent beads. Several different transitional stages leading to complete acrosomal exocytosis were classified, and we propose these represent true, functional intermediates since some of the AM proteins are orthologues of mouse proteins that bind the zona pellucida (ZP) of unfertilized eggs. In addition, we present evidence that implicates acrosin in the proteolytic processing of AM50 during AM disassembly. Thus, we propose that the transitional states of acrosomal exocytosis involve early binding of AM proteins to the ZP (by what visually appear to be “acrosome‐intact” sperm), maintenance of ZP binding that coincides with the progressive exposure of AM proteins, and gradual proteolytic disassembly of the AM to allow sperm movement through the ZP. We feel this “transitional states” model provides a more refined view of acrosomal function that supports a move away from the widely held, overly simplistic, and binary “acrosome‐reaction” model, and embraces a more dynamic view of acrosomal exocytosis that involves intermediate stages of the secretory process in ZP binding and penetration. Mol. Reprod. Dev. 78:930–941, 2011. © 2011 Wiley Periodicals, Inc.