Mesenchymal Stem Cells Improve Wound Healing In Vivo via Early Activation of Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor
- Title
- Mesenchymal Stem Cells Improve Wound Healing In Vivo via Early Activation of Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor
- Author
- 이장현
- Keywords
- Mesenchymal Stem Cells; Wound Healing
- Issue Date
- 2011-06
- Publisher
- KOREAN ACADEMY OF MEDICAL SCIENCE
- Citation
- JOURNAL OF KOREAN MEDICAL SCIENCE, 2011, 26(6), P.726-733
- Abstract
- We investigated the effects of mesenchymal stem cells (MSCs) on wound healing using a three-dimensional (3D) collagen gel scaffold. Three circular full-thickness skin defects were created on the back of Sprague-Dawley rats. One site was covered with a 3D collagen gel containing 2 × 106 MSCs (MSCs+/3D collagen+). Another site was replaced with a 3D collagen gel without MSCs and the third site was left empty. The wound size was significantly reduced in the MSCs+/3D collagen+ sites. MSCs+/3D collagen+ sites exhibited the most neovascularization. FISH showed that Y-chromosome possessing cells were found within the dermis of MSCs+/3D collagen+ sites. Gelatin zymography revealed that the most intense expression of MMP-9 was detected early in the MSCs+/3D collagen+ sites. Our results indicate that MSCs upregulate the early expression of MMP-9 which induces the early mobilization of VEGF. Thus, MSCs appear to accelerate significantly wound healing via early activation of MMP-9 and VEGF.
- URI
- https://synapse.koreamed.org/DOIx.php?id=10.3346/jkms.2011.26.6.726http://hdl.handle.net/20.500.11754/66709
- ISSN
- 1011-8934
- DOI
- 10.3346/jkms.2011.26.6.726
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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- jkms-26-726.pdfDownload
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