Functional Characterization of Exonic Variants of the PPARGC1B Gene in Coregulation of Estrogen Receptor Alpha
- Title
- Functional Characterization of Exonic Variants of the PPARGC1B Gene in Coregulation of Estrogen Receptor Alpha
- Author
- 정일엽
- Keywords
- SINGLE-NUCLEOTIDE POLYMORPHISMS; COACTIVATOR 1-BETA PGC-1-BETA; TYPE-2 DIABETES-MELLITUS; SMOOTH-MUSCLE; AIRWAY HYPERRESPONSIVENESS; TRANSCRIPTION FACTORS; SKELETAL-MUSCLE; MENSTRUAL-CYCLE; DOWN-REGULATION; IN-VIVO
- Issue Date
- 2016-05
- Publisher
- MARY ANN LIEBERT
- Citation
- DNA AND CELL BIOLOGY, v. 35, NO 7, Page. 314-321
- Abstract
- Peroxisome proliferator-activated receptor gamma coactivator 1 beta (PPARGC1B) is a coactivator of estrogen receptor (ER)alpha and ER beta. We previously demonstrated a significant association between a variant of exon 5 of the PPARGC1B gene (+102525 G˃A, R265Q) and airway hyperreactivity (AHR). The aims of the study were to evaluate the genetic effects of variants of the PPARGC1B gene on the function of ERs. PPARGC1B +102525G and A gene constructs were generated using PCR and cloned into a pCMV4 promoter vector. A luciferase reporter assay was undertaken in 293T cells cotransfected with one of the PPARGC1B +102525G˃A constructs, ER alpha, and an estrogen response element (ERE) containing a luciferase construct after treatment with 17 beta-estradiol. According to the luciferase reporter assay, the +102525A allele showed higher ER alpha activity than the +102525G allele in response to stimulation with 17 beta-estradiol. In addition, the interaction between ER alpha and PPARGC1B was evaluated by coprecipitation assay. Human influenza hemagglutinin-tagged PPARGC1B co-precipitated more intensely with ER alpha in the +102525A than the +102525G construct after 17 beta estradiol treatment. The variant +102525A allele enhances the activity of ER alpha to a greater degree than the +102525G allele of PPARGC1B.
- URI
- https://www.liebertpub.com/doi/10.1089/dna.2015.3195http://hdl.handle.net/20.500.11754/64737
- ISSN
- 1044-5498; 1557-7430
- DOI
- 10.1089/dna.2015.3195
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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