Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정일엽 | - |
dc.date.accessioned | 2018-04-05T06:31:05Z | - |
dc.date.available | 2018-04-05T06:31:05Z | - |
dc.date.issued | 2016-05 | - |
dc.identifier.citation | DNA AND CELL BIOLOGY, v. 35, NO 7, Page. 314-321 | en_US |
dc.identifier.issn | 1044-5498 | - |
dc.identifier.issn | 1557-7430 | - |
dc.identifier.uri | https://www.liebertpub.com/doi/10.1089/dna.2015.3195 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/64737 | - |
dc.description.abstract | Peroxisome proliferator-activated receptor gamma coactivator 1 beta (PPARGC1B) is a coactivator of estrogen receptor (ER)alpha and ER beta. We previously demonstrated a significant association between a variant of exon 5 of the PPARGC1B gene (+102525 G˃A, R265Q) and airway hyperreactivity (AHR). The aims of the study were to evaluate the genetic effects of variants of the PPARGC1B gene on the function of ERs. PPARGC1B +102525G and A gene constructs were generated using PCR and cloned into a pCMV4 promoter vector. A luciferase reporter assay was undertaken in 293T cells cotransfected with one of the PPARGC1B +102525G˃A constructs, ER alpha, and an estrogen response element (ERE) containing a luciferase construct after treatment with 17 beta-estradiol. According to the luciferase reporter assay, the +102525A allele showed higher ER alpha activity than the +102525G allele in response to stimulation with 17 beta-estradiol. In addition, the interaction between ER alpha and PPARGC1B was evaluated by coprecipitation assay. Human influenza hemagglutinin-tagged PPARGC1B co-precipitated more intensely with ER alpha in the +102525A than the +102525G construct after 17 beta estradiol treatment. The variant +102525A allele enhances the activity of ER alpha to a greater degree than the +102525G allele of PPARGC1B. | en_US |
dc.description.sponsorship | This study was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2013R1A2A2A01068077) and by a research grant from the Soonchunhyang University. The DNA samples were generously provided by the Soonchunhyang University Bucheon Hospital Biobank, a member of the National Biobank of Korea, supported by the Ministry of Health, Welfare and Family Affairs. | en_US |
dc.language.iso | en | en_US |
dc.publisher | MARY ANN LIEBERT | en_US |
dc.subject | SINGLE-NUCLEOTIDE POLYMORPHISMS | en_US |
dc.subject | COACTIVATOR 1-BETA PGC-1-BETA | en_US |
dc.subject | TYPE-2 DIABETES-MELLITUS | en_US |
dc.subject | SMOOTH-MUSCLE | en_US |
dc.subject | AIRWAY HYPERRESPONSIVENESS | en_US |
dc.subject | TRANSCRIPTION FACTORS | en_US |
dc.subject | SKELETAL-MUSCLE | en_US |
dc.subject | MENSTRUAL-CYCLE | en_US |
dc.subject | DOWN-REGULATION | en_US |
dc.subject | IN-VIVO | en_US |
dc.title | Functional Characterization of Exonic Variants of the PPARGC1B Gene in Coregulation of Estrogen Receptor Alpha | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 35 | - |
dc.identifier.doi | 10.1089/dna.2015.3195 | - |
dc.relation.page | 314-321 | - |
dc.relation.journal | DNA AND CELL BIOLOGY | - |
dc.contributor.googleauthor | Chang, Hun Soo | - |
dc.contributor.googleauthor | Lee, Shin-Hwa | - |
dc.contributor.googleauthor | Lee, Jong-Uk | - |
dc.contributor.googleauthor | Park, Jong Sook | - |
dc.contributor.googleauthor | Chung, Il Yup | - |
dc.contributor.googleauthor | Park, Choon-Sik | - |
dc.relation.code | 2016000980 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | iychu | - |
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