Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis
- Title
- Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis
- Author
- 황승용
- Keywords
- animal; article; cell separation; chemotaxis; cytology; Institute for Cancer Research mouse; microfluidic analysis; spermatozoon motility
- Issue Date
- 2012-01
- Publisher
- The Japan Society for Analytical Chemistry
- Citation
- Analytical sciences, 2012, 28(1), P.27-32
- Abstract
- We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection.
- URI
- https://www.jstage.jst.go.jp/article/analsci/28/1/28_1_27/_articlehttp://hdl.handle.net/20.500.11754/52786
- ISSN
- 0910-6340; 1348-2246
- DOI
- 10.2116/analsci.28.27
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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