Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황승용 | - |
dc.date.accessioned | 2018-03-27T00:20:35Z | - |
dc.date.available | 2018-03-27T00:20:35Z | - |
dc.date.issued | 2012-01 | - |
dc.identifier.citation | Analytical sciences, 2012, 28(1), P.27-32 | en_US |
dc.identifier.issn | 0910-6340 | - |
dc.identifier.issn | 1348-2246 | - |
dc.identifier.uri | https://www.jstage.jst.go.jp/article/analsci/28/1/28_1_27/_article | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/52786 | - |
dc.description.abstract | We present a novel method for the separation of progressive motile sperm from non-progressive motile and immotile sperm. This separation was accomplished by inducing chemotaxis along a longitudinal chemical gradient in a microchip composed of a biocompatible polydimethysiloxane layer and a glass substrate. In a preliminary experiment using fluorescent rhodamine B as a marker, we verified that a chemical gradient was generated by diffusion within the microchannel. We used acetylcholine as a chemoattractant to evaluate the chemotactic response of sperm. We tested the response to a 1/2 to 1/64 dilution series of acetylcholine. The results of a mouse sperm chemotaxis assay showed that progressive motile sperm swam predominantly toward the outlet at an optimal chemical gradient of 0.625 (mg/ml)/mm of acetylcholine. This device provides a convenient, disposable, and high-throughput platform that could function as a progressive motile sperm sorter for potential use in intracytoplasmic sperm injection. | en_US |
dc.description.sponsorship | This work was supported by a Korea Research Foundation grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-311-D00326). | en_US |
dc.language.iso | en | en_US |
dc.publisher | The Japan Society for Analytical Chemistry | en_US |
dc.subject | animal | en_US |
dc.subject | article | en_US |
dc.subject | cell separation | en_US |
dc.subject | chemotaxis | en_US |
dc.subject | cytology | en_US |
dc.subject | Institute for Cancer Research mouse | en_US |
dc.subject | microfluidic analysis | en_US |
dc.subject | spermatozoon motility | en_US |
dc.title | Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis | en_US |
dc.type | Article | en_US |
dc.relation.volume | 28 | - |
dc.identifier.doi | 10.2116/analsci.28.27 | - |
dc.relation.page | 27-32 | - |
dc.relation.journal | ANALYTICAL SCIENCES | - |
dc.contributor.googleauthor | KO, Yong-Jun | - |
dc.contributor.googleauthor | MAENG, Joon-Ho | - |
dc.contributor.googleauthor | LEE, Byung-Chul | - |
dc.contributor.googleauthor | LEE, Seungwoo | - |
dc.contributor.googleauthor | HWANG, Seung Yong | - |
dc.contributor.googleauthor | AHN, Yoomin | - |
dc.relation.code | 2012200601 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | syhwang | - |
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