Complete mitochondrial genome of the marine polychaete, Perinereis nuntia (Polychaeta, Nereididae)

Abstract

The main components of the mitochondrial genome of the marine polychaete, Perinereis nuntia, were initially obtained from the assembled expressed sequence tags by GS-FLX sequencer. After several cycles of polymerase chain reaction with genomic DNA, the complete mitochondrial genome of P. nuntia was obtained with 15,817 bp in length including 13 protein-coding genes (PCGs), two rRNA genes, and 22 tRNA genes with the same contents and order as shown in previous studies on other annelids. Some PCGs shared nucleotides with neighboring genes, and all 13 PCGs started with ATG as a start codon. The base composition of the mitochondrial genome of the polychaete, P. nuntia, showed high A+T (65.1%) and strong anti-G bias in their 3rd position (7.09%) of 13 PCGs.