Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이재성 | - |
dc.date.accessioned | 2018-03-26T03:08:01Z | - |
dc.date.available | 2018-03-26T03:08:01Z | - |
dc.date.issued | 2013-03 | - |
dc.identifier.citation | Aquatic Toxicology, 2013, 132, P.182-189 | en_US |
dc.identifier.issn | 0166-445X | - |
dc.identifier.issn | 1879-1514 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0166445X13000519 | - |
dc.description.abstract | The copepod, Tigriopus japonicus is an important model for toxicity testing. However, no attempt has been made in analyzing the effect of toxicants at the level of the ROS-mediated signal transduction pathway. To understand copper-induced cytotoxicity at the molecular level, we employed several cellular and biochemical assays after exposure to copper, and found a significant induction of enzyme activities of antioxidant proteins with increased intracellular reactive oxygen species (ROS) as well as an increase of TUNEL-positive cells, but a decrease of BrdU-positive cells. In addition, several important genes such as p38 MAPK, antioxidant-related genes, Hsps, and apoptosis-related genes were significantly modulated by copper exposure. Taken together, we suggest that copper-induced cytotoxicity is mediated by the formation of intracellular ROS and oxidative stress in T. japonicus. Whole body biochemical assays such as TUNEL- and BrdU-assay will provide a better understanding of cellular responses such as apoptosis and cell death upon cytotoxic exposure of copper in T. japonicus. | en_US |
dc.description.sponsorship | We thank Dr. Hans-U. Dahms for his comments on the manuscript, and also thank two anonymous reviewers on their valuable comments. This work was supported by a grant of MOMAF (2013; grant no. 201200000002729) funded to Jae-Seong Lee. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V | en_US |
dc.subject | Tigriopus japonicus | en_US |
dc.subject | Copper | en_US |
dc.subject | Cytotoxicity | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | TUNEL assay | en_US |
dc.subject | BrdU assay | en_US |
dc.subject | p38 MAPK | en_US |
dc.title | Copper induces apoptotic cell death through reactive oxygen species-triggered oxidative stress in the intertidal copepod Tigriopus japonicus | en_US |
dc.type | Article | en_US |
dc.relation.volume | 132 | - |
dc.identifier.doi | 10.1016/j.aquatox.2013.02.013 | - |
dc.relation.page | 182-189 | - |
dc.relation.journal | AQUATIC TOXICOLOGY | - |
dc.contributor.googleauthor | Rhee, JaeSung | - |
dc.contributor.googleauthor | Yu, InTag | - |
dc.contributor.googleauthor | Kim, BoMi | - |
dc.contributor.googleauthor | Jeong, ChangBum | - |
dc.contributor.googleauthor | Lee, KyunWoo | - |
dc.contributor.googleauthor | Kim, MinJung | - |
dc.contributor.googleauthor | Lee, SuJae | - |
dc.contributor.googleauthor | Park, GyungSoo | - |
dc.contributor.googleauthor | Lee, JaeSeong | - |
dc.relation.code | 2013008990 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | JSLee2 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.