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Characterization of optimized production, purification and application of laccase from Ganoderma lucidum

Title
Characterization of optimized production, purification and application of laccase from Ganoderma lucidum
Author
Klaus Heese
Keywords
Ganoderma lucidum; Laccase; Ethanol; Tamarind shell; Acid Fast Red A; Methyl Violet 2B; Remazol Yellow G
Issue Date
2013-01
Publisher
Elsevier Science B.V
Citation
Biochemical Engineering Journal, 2013, 70, P.106-114
Abstract
We show for the first-time Ganoderma lucidum laccase enzyme production using medium containing 3% (v/v) ethanol, which enhanced the enzyme production up to 14.1 folds. A more than 400-folds increase could be achieved if grown in the presence of the novel lignocellulosic biomass tamarind shell plus ethanol (3%, v/v), CuSO4 (0.4 mM) and gallic acid (1 mM). A 38.3 kDa laccase enzyme was purified from the initial protein preparation with an overall yield of 32% using Sephadex G-100 and DEAE-cellulose column chromatography. The enzyme was identified through MALDI-TOF/TOF tandem mass spectrometry (MS/MS) as G. lucidum laccase-3. This enzyme exerted its optimal activity at a pH of 5 and a temperature of 55 degrees C with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) as an ideal substrate. The catalytic efficiencies (k(cat)/K-m) determined for ABTS and guaiacol were 11.5 x 10(5) and 3.9 x 10(5)s(-1) M-1, respectively. The G. lucidum laccase decolorized various textile dyes and industrial textile dye effluent up to 90% and 97%, respectively. (C) 2012 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S1369703X12002859?via%3Dihubhttp://hdl.handle.net/20.500.11754/51940
ISSN
1369-703X; 1873-295X
DOI
10.1016/j.bej.2012.10.007
Appears in Collections:
GRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S](의생명공학전문대학원) > ETC
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