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dc.contributor.authorKlaus Heese-
dc.date.accessioned2018-03-25T06:11:14Z-
dc.date.available2018-03-25T06:11:14Z-
dc.date.issued2013-01-
dc.identifier.citationBiochemical Engineering Journal, 2013, 70, P.106-114en_US
dc.identifier.issn1369-703X-
dc.identifier.issn1873-295X-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S1369703X12002859?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/51940-
dc.description.abstractWe show for the first-time Ganoderma lucidum laccase enzyme production using medium containing 3% (v/v) ethanol, which enhanced the enzyme production up to 14.1 folds. A more than 400-folds increase could be achieved if grown in the presence of the novel lignocellulosic biomass tamarind shell plus ethanol (3%, v/v), CuSO4 (0.4 mM) and gallic acid (1 mM). A 38.3 kDa laccase enzyme was purified from the initial protein preparation with an overall yield of 32% using Sephadex G-100 and DEAE-cellulose column chromatography. The enzyme was identified through MALDI-TOF/TOF tandem mass spectrometry (MS/MS) as G. lucidum laccase-3. This enzyme exerted its optimal activity at a pH of 5 and a temperature of 55 degrees C with ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) as an ideal substrate. The catalytic efficiencies (k(cat)/K-m) determined for ABTS and guaiacol were 11.5 x 10(5) and 3.9 x 10(5)s(-1) M-1, respectively. The G. lucidum laccase decolorized various textile dyes and industrial textile dye effluent up to 90% and 97%, respectively. (C) 2012 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThe authors thank the University Grants Commission for providing the scholarship to T.M. through in Science for Meritorious Students and Dr. R. Rengasamy, CAS in Botany, University of Madras, for providing the adequate laboratory facility to carry out research. This study was supported by the Institute of Advanced Studies, Nanyang Technological University and by the Hanyang University.en_US
dc.language.isoenen_US
dc.publisherElsevier Science B.Ven_US
dc.subjectGanoderma lucidumen_US
dc.subjectLaccaseen_US
dc.subjectEthanolen_US
dc.subjectTamarind shellen_US
dc.subjectAcid Fast Red Aen_US
dc.subjectMethyl Violet 2Ben_US
dc.subjectRemazol Yellow Gen_US
dc.titleCharacterization of optimized production, purification and application of laccase from Ganoderma lucidumen_US
dc.typeArticleen_US
dc.relation.volume70-
dc.identifier.doi10.1016/j.bej.2012.10.007-
dc.relation.page106-114-
dc.relation.journalBIOCHEMICAL ENGINEERING JOURNAL-
dc.contributor.googleauthorManavalan, Tamilvendan-
dc.contributor.googleauthorManavalan, Arulmani-
dc.contributor.googleauthorThangavelu, Kalaichelvan P.-
dc.contributor.googleauthorHeese, Klaus-
dc.relation.code2013001106-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL OF BIOMEDICAL SCIENCE AND ENGINEERING[S]-
dc.identifier.pidklaus-
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