Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 노재숙 | - |
dc.date.accessioned | 2018-03-23T08:58:08Z | - |
dc.date.available | 2018-03-23T08:58:08Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | JOURNAL OF OVARIAN RESEARCH, 권: 7 | en_US |
dc.identifier.issn | 1757-2215 | - |
dc.identifier.uri | https://ovarianresearch.biomedcentral.com/articles/10.1186/s13048-014-0118-y | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/51671 | - |
dc.description.abstract | Background: The important role of WT1 in early folliculogenesis was evident from its restricted expression pattern in immature follicles and from its involvement in transcriptional control of inhibin-a and FSH receptor. There is also considerable evidence that WT1 is a potent inhibitor of apoptotic cell death in the developing kidney and male germ cells, suggesting that it could play a role in the regulation of follicle survival. Therefore, we evaluated if WT1 involves in cell survival of granulosa cells (GCs) during the FSH-independent stage. Methods: GCs were obtained from small preantral follicles of immature rat ovary. Bax and bcl-2 mRNA and protein levels in GCs transfected with WT1 (-KTS) or WT1 (+KTS) were analyzed by Real-time RT-PCR and immune-blotting analysis. Cell viability was measured with MTT assays and apoptosis was analyzed with caspase 3/7 activity and TUNEL assay. The mechanism by which WT1 regulates Bax expression was investigated using Bax promoter-luciferase reporter assay and ChIP assays from GCs. Results: Here, we showed that WT1 (-KTS) suppressed endogenous Bax transcript and protein expression, and this inhibition resulted from direct binding of WT1 in the Bax promoter region in vivo. In addition, anti-apoptotic effects of WT1 (-KTS) were demonstrated based on MTT assays, a sensitive bioluminescence caspase 3/7 assay and TUNEL assays. On the other hand, WT1 has no role on bcl-2 expression in GCs. Conclusion: These findings suggest that activation of WT1 is necessary for maintenance of GC survival during early stage of follicles and WT1 can play a role in protecting apoptosis through the regulation of upstream activator (Bax), as well as through regulation of downstream effecter (caspases 3 and 7). | en_US |
dc.description.sponsorship | This work was supported by National Research Foundation of Korea Grant funded by the Korean Government [NRF-2014R1A1A2053601, and 2013R1A1A2057826]. | en_US |
dc.language.iso | en | en_US |
dc.publisher | BIOMED CENTRAL LTD | en_US |
dc.subject | WT1 | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | Bax | en_US |
dc.subject | bcl-2 | en_US |
dc.subject | Caspase | en_US |
dc.subject | Granulosa cell | en_US |
dc.subject | Rat | en_US |
dc.title | Wilms' tumor suppressor gene (WT1) suppresses apoptosis by transcriptionally downregulating BAX expression in immature rat granulosa cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 118 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1186/s13048-014-0118-y | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | JOURNAL OF OVARIAN RESEARCH | - |
dc.contributor.googleauthor | Park, Minji | - |
dc.contributor.googleauthor | Choi, Yuri | - |
dc.contributor.googleauthor | Choi, Hyeonhae | - |
dc.contributor.googleauthor | Roh, Jaesook | - |
dc.relation.code | 2014034117 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | rohjaesook | - |
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