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dc.contributor.author전병훈-
dc.date.accessioned2018-03-23T07:53:19Z-
dc.date.available2018-03-23T07:53:19Z-
dc.date.issued2014-11-
dc.identifier.citationLetters in Applied Microbiology, 2014, 59(5), P.535-541en_US
dc.identifier.issn0266-8254-
dc.identifier.issn1472-765X-
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/full/10.1111/lam.12311-
dc.description.abstractThis study investigated the effect of pesticide chlorpyrifos (CP) on a freshwater cyanobacterium Chroococcus turgidus NTMS12. The changes in chlorophyll-a, proline, superoxide dismutase (SOD), catalase (CAT) activities and fatty acid composition of the test organism were analysed. Organism was grown at 6, 9 and 12mgl(-1) of CP, and based on the chlorophyll-a content, 6mgl(-1) of CP was found to be the tolerable concentration. Hence, 6mgl(-1) of CP was taken to evaluate the concentration of proline and activities of SOD and CAT at 48-h exposure. The changes in the fatty acid profile were analysed after 7days of exposure. Upon pesticide exposure, increased concentration of proline and activities of SOD and CAT were found. Significant changes in fatty acid profile have also been observed. However, polyunsaturated fatty acid content was decreased in treated cultures when compared with the untreated control. Changes in biochemical activities indicate that cyanobacteria C.turgidus NTMS12 undergo adaptive changes against CP-induced oxidative stress. Significance and Impact of the StudyThe fish pathogenic bacteria of genus Aeromonas, Vibrio, Edwardsiella and Streptococcus frequently cause severe outbreaks of diseases in cultured fish, and the genus-specific multiplex PCR assay developed in this study can detect the bacteria of the four genera when present in the samples either alone or mixed. The mPCR assay is expected to identify the causative agents more efficiently than uniplex PCR or species-specific multiplex PCR for clinical diagnosis, resulting in the earlier implementation of control measures. This mPCR assay provides a rapid, specific and sensitive tool for the detection or identification of common fish pathogenic bacteria in aquaculture practice.en_US
dc.description.sponsorshipM.S. acknowledges the award of University Grants Commission-Rajiv Gandhi National Fellowship (UGC-RGNF), Goverment of India and financial support from Hanyang University, South Korea. The financial support from the Department of Biotechnology (DBT), Government of India, is gratefully acknowledged.en_US
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.subjectcatalaseen_US
dc.subjectchlorpyrifosen_US
dc.subjectChroococcus turgidusen_US
dc.subjectgas chromatographyen_US
dc.subjectprolineen_US
dc.subjectsuperoxide dismutaseen_US
dc.titleChlorpyrifos-induced changes in the antioxidants and fatty acid compositions of Chroococcus turgidus NTMS12en_US
dc.typeArticleen_US
dc.relation.no5-
dc.relation.volume59-
dc.identifier.doi10.1111/lam.12311-
dc.relation.page535-541-
dc.relation.journalLETTERS IN APPLIED MICROBIOLOGY-
dc.relation.code2014035402-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF ENGINEERING[S]-
dc.sector.departmentDEPARTMENT OF EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING-
dc.identifier.pidbhjeon-
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COLLEGE OF ENGINEERING[S](공과대학) > EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING(자원환경공학과) > Articles
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