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Comparison of extraction phases for a two-phase culture of a recombinant E-coli producing retinoids

Title
Comparison of extraction phases for a two-phase culture of a recombinant E-coli producing retinoids
Author
김진웅
Keywords
Escherichia coli; In situ extraction; Retinoids; Two-phase culture
Issue Date
2014-03
Publisher
Springer Science + Business Media
Citation
Biotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology,v.36,no.3 2014년, pp.497 - 505
Abstract
To prevent degradation of intracellular retinoids through in situ extraction from the cells, a two-phase culture system was performed. Several organic solvents, including n-alkanes, mineral oils and cosmetic raw materials, were applied as the extraction phase. Of the n-alkanes, n-decane had the highest retinoid production as 134 mg/l after 72 h. For mineral oil, light and heavy mineral oil gave retinoid productions of 158 and 174 mg/l after 96 h, respectively. Of other materials, isopropyl myristate gave the highest retinoid production of 181 mg/l. These results indicate that many types of oils can be applied for retinoid production, and optimization of the in situ extraction process will lead to further improve of economical production for the industrial purpose.
URI
https://link.springer.com/article/10.1007%2Fs10529-013-1385-1http://hdl.handle.net/20.500.11754/51299
ISSN
0141-5492; 1573-6776
DOI
10.1007/s10529-013-1385-1
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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