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dc.contributor.author김진웅-
dc.date.accessioned2018-03-23T05:37:01Z-
dc.date.available2018-03-23T05:37:01Z-
dc.date.issued2014-03-
dc.identifier.citationBiotechnology letters. : a monthly journal for the rapid communication of results and developments in all aspects of biotechnology,v.36,no.3 2014년, pp.497 - 505en_US
dc.identifier.issn0141-5492-
dc.identifier.issn1573-6776-
dc.identifier.urihttps://link.springer.com/article/10.1007%2Fs10529-013-1385-1-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/51299-
dc.description.abstractTo prevent degradation of intracellular retinoids through in situ extraction from the cells, a two-phase culture system was performed. Several organic solvents, including n-alkanes, mineral oils and cosmetic raw materials, were applied as the extraction phase. Of the n-alkanes, n-decane had the highest retinoid production as 134 mg/l after 72 h. For mineral oil, light and heavy mineral oil gave retinoid productions of 158 and 174 mg/l after 96 h, respectively. Of other materials, isopropyl myristate gave the highest retinoid production of 181 mg/l. These results indicate that many types of oils can be applied for retinoid production, and optimization of the in situ extraction process will lead to further improve of economical production for the industrial purpose.en_US
dc.description.sponsorshipThis work was supported by a grant (NRF-2010-C1AAA001-0029084) from the National Research Foundation, the Intelligent Synthetic Biology Center of Global Frontier Project funded by the MEST (2011-0031964), and a grant from the Next-Generation BioGreen 21 Program (SSAC, Grant#: PJ009522003), RDA, Korea.en_US
dc.language.isoenen_US
dc.publisherSpringer Science + Business Mediaen_US
dc.subjectEscherichia colien_US
dc.subjectIn situ extractionen_US
dc.subjectRetinoidsen_US
dc.subjectTwo-phase cultureen_US
dc.titleComparison of extraction phases for a two-phase culture of a recombinant E-coli producing retinoidsen_US
dc.typeArticleen_US
dc.relation.volume36-
dc.identifier.doi10.1007/s10529-013-1385-1-
dc.relation.page497-505-
dc.relation.journalBIOTECHNOLOGY LETTERS-
dc.contributor.googleauthorJang, Hui-Jeong-
dc.contributor.googleauthorHa, Bo-Kyung-
dc.contributor.googleauthorKim, Jin-Woong-
dc.contributor.googleauthorJung, Kyung-Hwa-
dc.contributor.googleauthorAhn, Ji-yoon-
dc.contributor.googleauthorYoon, Sang-Hwal-
dc.contributor.googleauthorKim, Seon-Won-
dc.relation.code2014026241-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidkjwoong-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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