Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서혜명 | - |
dc.date.accessioned | 2018-03-19T05:01:45Z | - |
dc.date.available | 2018-03-19T05:01:45Z | - |
dc.date.issued | 2016-01 | - |
dc.identifier.citation | MOLECULAR NEUROBIOLOGY, v. 53, No. 1, Page. 95-108 | en_US |
dc.identifier.issn | 0893-7648 | - |
dc.identifier.issn | 1559-1182 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007/s12035-014-8989-x | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/48882 | - |
dc.description.abstract | Dysfunction of growth factor (GF) activities contributes to the decline and death of neurons during aging and in neurodegenerative diseases. In addition, neurons become more resistant to GF signaling with age. Micro (mi)RNAs are posttranscriptional regulators of gene expression that may be crucial to age- and disease-related changes in GF functions. MiR-126 is involved in regulating insulin/IGF-1/phosphatidylinositol-3-kinase (PI3K)/AKT and extracellular signal-regulated kinase (ERK) signaling, and we recently demonstrated a functional role of miR-126 in dopamine neuronal cell survival in models of Parkinson's disease (PD)-associated toxicity. Here, we show that elevated levels of miR-126 increase neuronal vulnerability to ubiquitous toxicity mediated by staurosporine (STS) or Alzheimer's disease (AD)-associated amyloid beta 1-42 peptides (A beta(1-42)). The neuroprotective factors IGF-1, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and soluble amyloid precursor protein alpha (sAPP alpha) could diminish but not abrogate the toxic effects of miR-126. In miR-126 overexpressing neurons derived from Tg6799 familial AD model mice, we observed an increase in A beta(1-42) toxicity, but surprisingly, both A beta(1-42) and miR-126 promoted neurite sprouting. Pathway analysis revealed that miR-126 overexpression downregulated elements in the GF/PI3K/AKT and ERK signaling cascades, including AKT, GSK-3 beta, ERK, their phosphorylation, and the miR-126 targets IRS-1 and PIK3R2. Finally, inhibition of miR-126 was neuroprotective against both STS and A beta(1-42) toxicity. Our data provide evidence for a novel mechanism of regulating GF/PI3K signaling in neurons by miR-126 and suggest that miR-126 may be an important mechanistic link between metabolic dysfunction and neurotoxicity in general, during aging, and in the pathogenesis of specific neurological disorders, including PD and AD. | en_US |
dc.description.sponsorship | We want to thank Dr. D. Trono (EPFL) for providing the third generation lentivirus vector system, Dr. A. Miyanohara (UCSD) for the Synapsin. GFP, and Dr. I. Verma for the CAG.NGF.GFP lentivirus construct. This research was supported by the National Institute of Health grants NS067335 (K.C.S.) and NS070577 (K.S.K.) and by grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (NRF Grants 2011-0030928, 2011-0030049, 2012-003338 to H.S.). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer | en_US |
dc.subject | MiR-126 | en_US |
dc.subject | Growth factors | en_US |
dc.subject | Neurotoxicity | en_US |
dc.subject | Neuroprotection | en_US |
dc.subject | PI3K/AKTsignaling | en_US |
dc.subject | A beta | en_US |
dc.subject | AMYLOID PRECURSOR PROTEIN | en_US |
dc.subject | A-BETA OLIGOMERS | en_US |
dc.subject | ALZHEIMERS-DISEASE | en_US |
dc.subject | PARKINSONS-DISEASE | en_US |
dc.subject | ENDOTHELIAL-CELLS | en_US |
dc.subject | GENE DELIVERY | en_US |
dc.subject | MOUSE MODEL | en_US |
dc.subject | IN-VIVO | en_US |
dc.subject | NEURODEGENERATIVE DISEASES | en_US |
dc.subject | TAU-PHOSPHORYLATION | en_US |
dc.title | MiR-126 Regulates Growth Factor Activities and Vulnerability to Toxic Insult in Neurons | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 53 | - |
dc.identifier.doi | 10.1007/s12035-014-8989-x | - |
dc.relation.page | 95-108 | - |
dc.relation.journal | MOLECULAR NEUROBIOLOGY | - |
dc.contributor.googleauthor | Kim, Woori | - |
dc.contributor.googleauthor | Noh, Haneul | - |
dc.contributor.googleauthor | Lee, Yenarae | - |
dc.contributor.googleauthor | Jeon, Jeha | - |
dc.contributor.googleauthor | Shanmugavadivu, Arthi | - |
dc.contributor.googleauthor | McPhie, Donna L. | - |
dc.contributor.googleauthor | Kim, Kwang-Soo | - |
dc.contributor.googleauthor | Cohen, Bruce M | - |
dc.contributor.googleauthor | Seo, Hyemyung | - |
dc.contributor.googleauthor | Sonntag, Kai C | - |
dc.relation.code | 2016000392 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MOLECULAR AND LIFE SCIENCE | - |
dc.identifier.pid | hseo | - |
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