Gene networking and inflammatory pathway analysis in a JMJD3 knockdown human monocytic cell line
- Title
- Gene networking and inflammatory pathway analysis in a JMJD3 knockdown human monocytic cell line
- Author
- 김승현
- Keywords
- JMJD3; NF-?B; THP-1 cells; IPA; gene networking
- Issue Date
- 2012-04
- Publisher
- John Wiley & Sons, Ltd
- Citation
- CELL BIOCHEMISTRY AND FUNCTION, 2012, 30(3), P.224-232
- Abstract
- JMJD3, a Jumonji C family histone demethylase, is induced by transcription factor, nuclear factor-kappa B (NF-?B), in response to various stimuli. JMJD3 is crucial for erasing histone-3 lysine-27 trimethylation (H3K27me3), a modification associated with transcriptional repression and is responsible for the activation of a diverse set of genes. Here, we identify the genes in human leukaemia monocyte (THP-1) human monocytic cells that are significantly affected by the stable knockdown (kd) of JMJD3. Global gene expression levels were detected in stable JMJD3 knockdown THP-1 cells and in tumor necrosis factor-alpha (TNF-a)-stimulated JMJD3-kd THP-1 cells by using a 12-plex NimbleGen human whole genome array. In addition, datasets were analysed by using Ingenuity Pathway Analysis. Stable knockdown of JMJD3 in THP-1 cells affected particularly in expression levels and in downstream effects on inflammatory signalling pathways. JMJD3 attenuation down-regulates various key genes in NF-?B, chemokine and CD40 signalling, and mostly affects inflammatory disease response molecules. In addition, chromatin immunoprecipitation revealed that JMJD3-kd could inhibit several NF-?B-regulated inflammatory genes by recruiting repressive histone-3 lysine-27 trimethylation to their promoters. Moreover, this study significantly highlights the connexion of NF-?B with JMJD3, which suggests an epigenetic regulation in different signalling pathways. Finally, this study establishes novel JMJD3 targets through Ingenuity Pathway Analysis. Copyright (C) 2012 John Wiley & Sons, Ltd.
- URI
- http://onlinelibrary.wiley.com/doi/10.1002/cbf.1839/abstract;jsessionid=691EC25C3E6E21A284E54361CDAFC3E9.f02t03http://hdl.handle.net/20.500.11754/46850
- ISSN
- 0263-6484
- DOI
- 10.1002/cbf.1839
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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