Highly sensitive detection of thrombin using SERS-based magnetic aptasensors

Title
Highly sensitive detection of thrombin using SERS-based magnetic aptasensors
Author
주재범
Keywords
Thrombin; Magnetic bead; Aptamer; Surface-enhanced Raman scattering; Aptasensor
Issue Date
2013-04
Publisher
Elsevier Science B.V., Amsterdam.
Citation
Biosensors & bioelectronics, April 2013, 47, P.62-67
Abstract
This paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (Au NPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA(15)) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA(29))-conjugated Au NPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection (LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27 pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease. (C) 2013 Elsevier B.V. All rights reserved.
URI
https://www.sciencedirect.com/science/article/pii/S0956566313001619?via%3Dihubhttp://hdl.handle.net/20.500.11754/44292
ISSN
0956-5663
DOI
10.1016/j.bios.2013.03.003
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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