Highly sensitive detection of thrombin using SERS-based magnetic aptasensors
- Title
- Highly sensitive detection of thrombin using SERS-based magnetic aptasensors
- Author
- 주재범
- Keywords
- Thrombin; Magnetic bead; Aptamer; Surface-enhanced Raman scattering; Aptasensor
- Issue Date
- 2013-04
- Publisher
- Elsevier Science B.V., Amsterdam.
- Citation
- Biosensors & bioelectronics, April 2013, 47, P.62-67
- Abstract
- This paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (Au NPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA(15)) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA(29))-conjugated Au NPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection (LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27 pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease. (C) 2013 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S0956566313001619?via%3Dihubhttp://hdl.handle.net/20.500.11754/44292
- ISSN
- 0956-5663
- DOI
- 10.1016/j.bios.2013.03.003
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML