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dc.contributor.author주재범-
dc.date.accessioned2018-03-09T06:28:51Z-
dc.date.available2018-03-09T06:28:51Z-
dc.date.issued2013-04-
dc.identifier.citationBiosensors & bioelectronics, April 2013, 47, P.62-67en_US
dc.identifier.issn0956-5663-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0956566313001619?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/44292-
dc.description.abstractThis paper reports a method of highly sensitive detection of thrombin using a surface-enhanced Raman scattering (SERS)-based magnetic aptasensor. Magnetic beads and gold nanoparticles (Au NPs) were used as supporting substrates and sensing probes, respectively. For this purpose, 15-mer thrombin-binding aptamers (TBA(15)) were immobilized onto the surface of magnetic beads, and then thrombin antigens and 29-mer thrombin-binding aptamer (TBA(29))-conjugated Au NPs were sequentially added for the formation of sandwich aptamer complexes. Quantitative analysis was performed by monitoring the intensity variation of a characteristic SERS signal of Raman reporter molecules. Because all of the reactions occur in solution, this SERS-based immunoassay technique can solve the diffusion-limited kinetic problems on a solid substrate. The limit of detection (LOD) of thrombin, determined by the SERS-based aptasensor, was estimated to be 0.27 pM. The proposed method is expected to be a good clinical tool for the diagnosis of a thrombotic disease. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipNational Research Foundation of Korea Ministry of Health & Welfare, Republic of Koreaen_US
dc.language.isoenen_US
dc.publisherElsevier Science B.V., Amsterdam.en_US
dc.subjectThrombinen_US
dc.subjectMagnetic beaden_US
dc.subjectAptameren_US
dc.subjectSurface-enhanced Raman scatteringen_US
dc.subjectAptasensoren_US
dc.titleHighly sensitive detection of thrombin using SERS-based magnetic aptasensorsen_US
dc.typeArticleen_US
dc.relation.volume47-
dc.identifier.doi10.1016/j.bios.2013.03.003-
dc.relation.page62-67-
dc.relation.journalBIOSENSORS & BIOELECTRONICS-
dc.contributor.googleauthorYoon, Jiyeon-
dc.contributor.googleauthorChoi, Namhyun-
dc.contributor.googleauthorKo, Juhui-
dc.contributor.googleauthorKim, Kihyung-
dc.contributor.googleauthorLee, Sangyeop-
dc.contributor.googleauthorChoo, Jaebum-
dc.relation.code2009201344-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidjbchoo-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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