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Cell-Based Dose Responses from Open-Well Microchambers

Title
Cell-Based Dose Responses from Open-Well Microchambers
Author
Jong Wook Hong
Keywords
DRUG DISCOVERY; MICROFLUIDIC DEVICES; IN-VIVO; ANTITUMOR-ACTIVITY; PERFUSION CULTURE; GENERATION; CYTOTOXICITY; BIOLOGY; SCALE; GRADIENTS
Issue Date
2013-04
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, April 2013, 85(10), P.5249-5254
Abstract
Cell-based assays play a critical role in discovery of new drugs and facilitating research in cancer, immunology, and stem cells. Conventionally, they are performed in Petri dishes, tubes, or well plates, using milliliters of reagents and thousands of cells to obtain one data point. Here, we are introducing a new platform to realize cell-based assay capable of increased throughput and greater sensitivity with a limited number of cells. We integrated an array of open-well microchambers into a gradient generation system. Consequently, cell-based dose responses were examined with a single device. We measured IC50 values of three cytotoxic chemicals, Triton X-100, H2O2 and cadmium chloride, as model compounds. The present system is highly suitable for the discovery of new drugs and studying the effect of chemicals on cell viability or mortality with limited samples and cells.
URI
http://dx.doi.org/10.1021/ac400743whttp://hdl.handle.net/20.500.11754/44167
ISSN
0003-2700
DOI
10.1021/ac400743w
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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