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dc.contributor.authorJong Wook Hong-
dc.date.accessioned2018-03-09T05:24:48Z-
dc.date.available2018-03-09T05:24:48Z-
dc.date.issued2013-04-
dc.identifier.citationANALYTICAL CHEMISTRY, April 2013, 85(10), P.5249-5254en_US
dc.identifier.issn0003-2700-
dc.identifier.urihttp://dx.doi.org/10.1021/ac400743w-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/44167-
dc.description.abstractCell-based assays play a critical role in discovery of new drugs and facilitating research in cancer, immunology, and stem cells. Conventionally, they are performed in Petri dishes, tubes, or well plates, using milliliters of reagents and thousands of cells to obtain one data point. Here, we are introducing a new platform to realize cell-based assay capable of increased throughput and greater sensitivity with a limited number of cells. We integrated an array of open-well microchambers into a gradient generation system. Consequently, cell-based dose responses were examined with a single device. We measured IC50 values of three cytotoxic chemicals, Triton X-100, H2O2 and cadmium chloride, as model compounds. The present system is highly suitable for the discovery of new drugs and studying the effect of chemicals on cell viability or mortality with limited samples and cells.en_US
dc.description.sponsorshipNational Institutes of Health (NIH) National Science Foundation (NSF CBET) Marine Bioprocess Research Center Ministry of Land, Transport and Maritime National Science Foundation Ministry of Education, Science and Technology of Koreaen_US
dc.language.isoenen_US
dc.publisherAMER CHEMICAL SOCen_US
dc.subjectDRUG DISCOVERYen_US
dc.subjectMICROFLUIDIC DEVICESen_US
dc.subjectIN-VIVOen_US
dc.subjectANTITUMOR-ACTIVITYen_US
dc.subjectPERFUSION CULTUREen_US
dc.subjectGENERATIONen_US
dc.subjectCYTOTOXICITYen_US
dc.subjectBIOLOGYen_US
dc.subjectSCALEen_US
dc.subjectGRADIENTSen_US
dc.titleCell-Based Dose Responses from Open-Well Microchambersen_US
dc.typeArticleen_US
dc.relation.no10-
dc.relation.volume85-
dc.identifier.doi10.1021/ac400743w-
dc.relation.page5249-5254-
dc.relation.journalANALYTICAL CHEMISTRY-
dc.contributor.googleauthorHamon, Morgan-
dc.contributor.googleauthorJambovane, Sachin-
dc.contributor.googleauthorBradley, Lauren-
dc.contributor.googleauthorKhademhosseini, Ali-
dc.contributor.googleauthorHong, Jong Wook-
dc.relation.code2013008836-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidjwh-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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