Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2018-03-09T00:26:19Z | - |
dc.date.available | 2018-03-09T00:26:19Z | - |
dc.date.issued | 2013-07 | - |
dc.identifier.citation | Inflammation Research, 62 , 7, 689 - 696 | en_US |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs00011-013-0621-4 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/43856 | - |
dc.description.abstract | To investigate whether the phenyl-thiophenyl propenone RK-I-123 suppresses interleukin-8 (IL-8) expression and activation of mitogen-activated protein kinases (MAPKs) and transcription factors (nuclear factor-kappa B [NF-kappa B] and activator protein-1 [AP-1]) by reducing reactive oxygen species (ROS) levels in Helicobacter pylori-infected gastric epithelial cells.Helicobacter pylori in Korean isolates, human gastric epithelial AGS cells.AGS cells pretreated with or without RK-I-123 were cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1.Reactive oxygen species and IL-8 levels were determined by dichlorofluorescein fluorescence and enzyme-linked immunosorbent assay. The IL-8 mRNA expression was analyzed by the real-time reverse transcription-polymerase chain reaction (RT-PCR). The MAPK and I kappa B alpha levels were determined by western blotting. The activation of NF-kappa B and AP-1 was determined by the electrophoretic mobility shift assay.Helicobacter pylori induced an increase in ROS and IL-8 expression and activation of MAPKs and transcription factors (NF-kappa B and AP-1) together with the degradation of I kappa B alpha in AGS cells, all of which were inhibited by RK-I-123.The RK-I-123 suppressed the H. pylori-induced IL-8 expression and activation of MAPKs, NF-kappa B, and AP-1 by reducing ROS levels in AGS cells. The RK-I-123 may be a potential candidate for the treatment of H. pylori-induced gastric inflammation. | en_US |
dc.description.sponsorship | This study was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0000811). H. Kim is grateful to the Brain Korea 21 Project of the College of Human Ecology at Yonsei University. | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER BASEL AG, PICASSOPLATZ 4, BASEL, 4052, SWITZERLAND | en_US |
dc.subject | Helicobacter pylori | en_US |
dc.subject | RK-I-123 | en_US |
dc.subject | IL-8 | en_US |
dc.subject | NF-kappa B | en_US |
dc.subject | Gastric epithelial cells | en_US |
dc.title | The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-kappa B and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells | en_US |
dc.type | Article | en_US |
dc.relation.volume | 62 | - |
dc.identifier.doi | 10.1007/s00011-013-0621-4 | - |
dc.relation.page | 689-696 | - |
dc.relation.journal | INFLAMMATION RESEARCH | - |
dc.contributor.googleauthor | Jang, Sung Hee | - |
dc.contributor.googleauthor | Cho, Soonok | - |
dc.contributor.googleauthor | Lee, Eung-Seok | - |
dc.contributor.googleauthor | Kim, Jung Mogg | - |
dc.contributor.googleauthor | Kim, Hyeyoung | - |
dc.relation.code | 2013010258 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
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