Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2018-03-06T06:11:34Z | - |
dc.date.available | 2018-03-06T06:11:34Z | - |
dc.date.issued | 2012-11 | - |
dc.identifier.citation | Digestive Diseases and Sciences, 2012, 57(11), P.2873-2882 | en_US |
dc.identifier.issn | 0163-2116 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs10620-012-2237-x | - |
dc.description.abstract | Deguelin, a naturally occurring rotenoid, is known to be an Akt inhibitor and to have an anti-tumor effect on several cancers. This study was performed to elucidate the effect of deguelin on apoptotic pathways related to NF-kappa B signaling in colon cancer cells and on the anti-tumor effect in colon cancer xenograft mice. We studied COLO 205 and HCT116 cells in the presence or absence of deguelin. NF-kappa B signaling was examined by real-time RT-PCR for interleukin (IL)-8, by Western blotting for I kappa B phosphorylation/degradation, and by the electrophoretic mobility shift assay. Cell death was determined by the MTT assay, and apoptosis by Annexin V-FITC staining and caspase-3 activity. We also assessed the expression of antiapoptotic and proapoptotic factors by use of RT-PCR. In colon cancer xenograft mice, we evaluated the effect of deguelin on inoculated tumor growth, and apoptotic index was measured by the in vivo TUNEL assay. Deguelin significantly inhibited IL-8 gene expression, I kappa B phosphorylation/degradation, and DNA binding activity of NF-kappa B in colon cancer cells. Deguelin induced cell death and apoptosis in colon cancer cells in a dose and time-dependent manner. Deguelin down-regulated expression of NF-kappa B-mediated antiapoptotic factors such as cFLIP, Bcl-2, and Bcl-X-L. In the colon cancer xenograft model, the volume of the tumor treated with deguelin was significantly lower than that of the control, and the apoptotic index for deguelin-treated mice was much higher. Deguelin might be a potential therapeutic agent for treatment of colorectal cancer. | en_US |
dc.description.sponsorship | This study was supported in part by grant no. 03-2009-0060 from the SNUH Research Fund. The authors thank Chi-Yeon Lim, Ph.D., for her assistance with statistics. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Springer | en_US |
dc.subject | Deguelin | en_US |
dc.subject | Colon cancer | en_US |
dc.subject | NF-kappa B | en_US |
dc.subject | Apoptosis | en_US |
dc.title | Deguelin, an Akt Inhibitor, Down-Regulates NF-kappa B Signaling and Induces Apoptosis in Colon Cancer Cells and Inhibits Tumor Growth in Mice | en_US |
dc.type | Article | en_US |
dc.relation.no | 11 | - |
dc.relation.volume | 57 | - |
dc.identifier.doi | 10.1007/s10620-012-2237-x | - |
dc.relation.page | 2873-2882 | - |
dc.relation.journal | DIGESTIVE DISEASES AND SCIENCES | - |
dc.contributor.googleauthor | Kang, H.W. | - |
dc.contributor.googleauthor | Kim, J.M. | - |
dc.contributor.googleauthor | Cha, M.Y. | - |
dc.contributor.googleauthor | Jung, H.C. | - |
dc.contributor.googleauthor | Song, I.S. | - |
dc.contributor.googleauthor | Kim, J.S. | - |
dc.relation.code | 2012202588 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
dc.identifier.researcherID | 34771324000 | - |
dc.identifier.orcid | 0000-0002-6506-7519 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.