Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신경훈 | - |
dc.date.accessioned | 2018-02-28T05:28:36Z | - |
dc.date.available | 2018-02-28T05:28:36Z | - |
dc.date.issued | 2015-02 | - |
dc.identifier.citation | CHEMOSPHERE, v. 120, Page. 470-478 | en_US |
dc.identifier.issn | 0045-6535 | - |
dc.identifier.issn | 1879-1298 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0045653514010157 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/41198 | - |
dc.description.abstract | Superoxide dismutases (SODS) are important antioxidant enzymes whose expression levels are often used as biomarkers for oxidative stress. To investigate the biomarker potential of the monogonont rotifer Brachionus koreanus SOD genes, the full-length Cu/Zn-SOD (Bk-Cu/Zn-SOD) and Mn-SOD (Bk-Mn-SOD) genes were cloned from genomic DNA and characterized. All amino acid residues involved in the formation of tertiary structure and metal binding in Bk-Cu/Zn-SOD and Bk-Mn-SOD were highly conserved across species. Phylogenetic analysis revealed that Bk-Mn-SOD, in particular, was closely clustered with mitochondrial Mn-SOD. Transcript analysis after exposure to six different biocides (alachlor, chlorpyrifos, dimethoate, endosulfan, lindane, and molinate) revealed that the transcriptional level of Bk-Cu/Zn-SOD was significantly increased in a dose-dependent manner. In contrast, the level of Bk-Mn-SOD transcript was significantly increased compared with control cells in response to chlorpyrifos, endosulfan, and molinate at their no observed effect concentrations (NOECs). However, exposure to alachlor, chlorpyrifos, and molinate significantly reduced the enzymatic activity of total SOD protein, while a decreased pattern was observed in all biocide treatments. Taken together, these results indicate that exposure to waterborne environmental biocides induces the transcription of Bk-Cu/Zn-SOD and Bk-Mn-SOD, but inhibits the enzymatic activity of Bk-SODs. These results contribute to our understanding of the modes of action of oxidative stress-mediating biocides on rotifer. (C) 2014 Elsevier Ltd. All rights reserved. | en_US |
dc.description.sponsorship | We thank Prof. Hans-U. Dahms for his valuable comments on the manuscript and also thank two anonymous reviewers for their constructive comments on the previous manuscript. This work was supported by a Grant from the National Research Foundation (2012R1A1A2000970; South Korea) to Jae-Seong Lee. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | en_US |
dc.subject | Monogonont rotifer | en_US |
dc.subject | Brachionus koreanus | en_US |
dc.subject | Superoxide dismutase | en_US |
dc.subject | Biocides | en_US |
dc.subject | Enzymatic activity | en_US |
dc.subject | INDUCED OXIDATIVE STRESS | en_US |
dc.subject | DNA-DAMAGE | en_US |
dc.subject | EXPOSURE | en_US |
dc.subject | CALYCIFLORUS | en_US |
dc.subject | BIOMARKERS | en_US |
dc.subject | PESTICIDE | en_US |
dc.subject | COPPER | en_US |
dc.subject | GENE | en_US |
dc.subject | ECOTOXICOLOGY | en_US |
dc.subject | GLUTATHIONE | en_US |
dc.title | Modulated expression and enzymatic activity of the monogonont rotifer Brachionus koreanus Cu/Zn- and Mn-superoxide dismutase (SOD) in response to environmental biocides | en_US |
dc.type | Article | en_US |
dc.relation.volume | 120 | - |
dc.identifier.doi | 10.1016/j.chemosphere.2014.08.042 | - |
dc.relation.page | 470-478 | - |
dc.relation.journal | CHEMOSPHERE | - |
dc.contributor.googleauthor | Kim, Bo-Mi | - |
dc.contributor.googleauthor | Lee, Jin Wuk | - |
dc.contributor.googleauthor | Seo, Jung Soo | - |
dc.contributor.googleauthor | Shin, Kyung-Hoon | - |
dc.contributor.googleauthor | Rhee, Jae-Sung | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.relation.code | 2015003164 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MARINE SCIENCE AND CONVERGENCE ENGINEERING | - |
dc.identifier.pid | shinkh | - |
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