Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이재성 | - |
dc.date.accessioned | 2018-02-22T05:59:41Z | - |
dc.date.available | 2018-02-22T05:59:41Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Comparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology, 2012, 161(4), P.436-442 | en_US |
dc.identifier.issn | 1095-6433 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1095643312000074?via%3Dihub | - |
dc.description.abstract | Salinity is an important parameter that affects survival and metabolism in fish. In fish, pituitary growth hormone (GH) regulates physiological functions including adaptation to different salinity as well as somatic growth. GH is stimulated by growth hormone-releasing hormone (GHRH) and exerts its function via binding to growth hormone receptor (GHR). As Ktyptolebias marmoratus is a euryhaline fish, this species would be a useful model species for studying the adaptation to osmotic stress conditions. Here, we cloned GH, -GHR, somatolactin (SL), and somatolactin receptor (SLR) genes, and analyzed their expression patterns in different tissues and during early developmental stages by using real-time RT-PCR. We also further examined expression of them after acclimation to different salinity. Tissue distribution studies revealed that Km-GH and -SL mRNAs were remarkably expressed in brain and pituitary, whereas Km-GHR and -SLR mRNAs were predominantly expressed in liver, followed by gonad, muscle, pituitary, and brain. During embryonic developmental stages, the expression of their mRNA was increased at stage 3 (9 dpf). The Km-GH and -SL mRNA transcripts were constantly elevated until stage 5 (5 h post hatch), whereas Km-GHR and -SLR mRNA levels decreased at this stage. After we transferred K. marmoratus from control (12 psu) to hyper-osmotic condition (hypersea-water, HSW; 33 psu), Km-CH, -SL, and GHR mRNA levels were enhanced. In hypo-osmotic conditions like freshwater (FW), Km-GH and -SL expressions were modulated 24 h after exposure, and Km-SLR transcripts were significantly upregulated. This finding suggests that Km-GH and -SL may be involved in the osmoregulatory mechanism under hyper-osmotic as well as hypo-osmotic stress. This is the first report on transcriptional modulation and relationship of GH, GHR, SL, and SLR during early development and after salinity stress. This study will be helpful to a better understanding on molecular mechanisms of adaptation response to salt stress in euryhaline fish. | en_US |
dc.description.sponsorship | We thank Dr. Hans-U. Dahms for his comments on the manuscript. This work was supported by a grant of the National Research Foundation (2009-0078545) funded to Young-Mi Lee, and also partly supported by a grant of the National Korea Research Foundation (2006-J01902) funded to Jae-Seong Lee. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science INC | en_US |
dc.subject | Fish | en_US |
dc.subject | Kryptolebias marmoratus | en_US |
dc.subject | Growth hormone | en_US |
dc.subject | Growth hormone receptor | en_US |
dc.subject | Somatolactin | en_US |
dc.subject | Somatolactin receptor | en_US |
dc.subject | Salinity | en_US |
dc.title | Cloning of growth hormone, somatolactin, and their receptor mRNAs, their expression in organs, during development, and on salinity stress in the hermaphroditic fish, Kryptolebias marmoratus | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 161 | - |
dc.identifier.doi | 10.1016/j.cbpa.2012.01.004 | - |
dc.relation.page | 436-442 | - |
dc.relation.journal | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | - |
dc.contributor.googleauthor | Rhee, Jae-Sung | - |
dc.contributor.googleauthor | Kim, Bo-Mi | - |
dc.contributor.googleauthor | Seo, Jung Soo | - |
dc.contributor.googleauthor | Kim, Il-Chan | - |
dc.contributor.googleauthor | Lee, Young-Mi | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.contributor.googleauthor | 이재성 | - |
dc.contributor.googleauthor | 김보미 | - |
dc.contributor.googleauthor | 서정수 | - |
dc.contributor.googleauthor | 김일찬 | - |
dc.contributor.googleauthor | 이영미 | - |
dc.contributor.googleauthor | 이재승 | - |
dc.relation.code | 2012202143 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | JSLee2 | - |
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