Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이재성 | - |
dc.date.accessioned | 2018-02-22T05:48:26Z | - |
dc.date.available | 2018-02-22T05:48:26Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Mar 2012, 155(2), P.290-299 | en_US |
dc.identifier.issn | 1532-0456 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S153204561100189X?via%3Dihub | - |
dc.description.abstract | 8-Oxoguanine DNA glycosylase 1 (EC 3.2.2.23) is encoded by OGG1 gene and plays a key role in removing 8-oxo-7,8-dihydroguanine (8-oxoG) base in DNA lesion by reactive oxygen species (ROS). To identify and characterize OGG1 gene (TJ-OGG1) in the copepod Tigriopus japonicus, the full-length cDNA sequence, genomic structure, and promoter region was analyzed. In addition, to investigate transcriptional change of TJ-OGG1 mRNA under oxidative stress conditions, T. japonicus were exposed to environmental oxidative inducers, H2O2. UV-B, and heavy metals (Cd, Cu, and Zn), respectively. The full-length cDNA of TJ-OGG1 gene was 1708 bp in length, encoding 343 amino acid residues. The deduced amino acid sequences of TJ-OGG1 showed a 56% similarity with human. Two conserved motifs (HhH and PVD loop) and two conserved residues (lysine and aspartic acid) in active sites were also observed. TJ-OGG1 genome structure contained six exons and five introns and putative transcription factor binding sites such as Nrf-2, p53, ERE-half sites, and XRE were detected on the promoter region. TJ-OGG1 mRNA level was increased at approximately three-fold (P<0.05) at 1 mM and approximately 4-fold (P<0.01) at 10 mM of H2O2, respectively. UV-B enhanced the expression of TJ-OGG1 mRNA at 15 kJ/m(2) (P<0.05) and more (P<0.001). In a time-course experiment. TJ-OGG1 gene was highly transcribed within 12 h after exposure of 10 kJ/m(2) (P<0.01) and 20 kJ/m(2) (P<0.001). The expression of TJ-OGG1 mRNA after exposure to Cu and Cd for 96 h was significantly up-regulated at 0.1 mu g/L and then remarkably reduced in a dose-dependent manner. Their transcript levels did not change at low dose (0.1 and 1 mu g/L) but were dose-dependently down-regulated at high dose (10 and 100 mu g/L). These findings suggest that H2O2, UV-B, and heavy metals induce oxidative stress and generate oxidatively damaged DNA. Consequently, the enhanced TJ-OGG1 gene expression would be associated with active involvement of TJ-OGG1 gene in DNA repair process as a cellular protection mechanism. This is the first report on the cloning and characterization of OGG1 gene in aquatic animals. This study is helpful for a better understanding of the molecular mechanisms of cellular protection against various environmental oxidative stress inducers such as UV-B and heavy metals in aquatic invertebrates. | en_US |
dc.description.sponsorship | We thank Dr. Hans-U. Dahms for his comments on the manuscript. This work was supported by a grant of Eco-Technopia 21 (2010) funded to Jae-Seong Lee, and was partly supported by a research fund from the Korean Ministry of Land, Transport and Maritime Affairs (2010; PM56380: Oil Spill Environmental Impact Assessment and Environmental Restoration) funded to Jae-Seong Lee. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science INC. | en_US |
dc.subject | Copepod | en_US |
dc.subject | Tigriopus japonicus | en_US |
dc.subject | 8-oxoguanine DNA glycosylase 1 | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | Hydrogen peroxide | en_US |
dc.subject | UV-B | en_US |
dc.subject | Heavy metals | en_US |
dc.title | 8-Oxoguanine DNA glycosylase 1 (OGG1) from the copepod Tigriopus japonicus: Molecular characterization and its expression in response to UV-B and heavy metals | en_US |
dc.type | Article | en_US |
dc.relation.no | 2 | - |
dc.relation.volume | 155 | - |
dc.identifier.doi | 10.1016/j.cbpc.2011.09.010 | - |
dc.relation.page | 290-299 | - |
dc.relation.journal | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | - |
dc.contributor.googleauthor | Kim, Bo-Mi | - |
dc.contributor.googleauthor | Rhee, Jae-Sung | - |
dc.contributor.googleauthor | Seo, Jung Soo | - |
dc.contributor.googleauthor | Kim, Il-Chan | - |
dc.contributor.googleauthor | Lee, Young-Mi | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.contributor.googleauthor | 김보미 | - |
dc.contributor.googleauthor | 이재성 | - |
dc.contributor.googleauthor | 서정수 | - |
dc.contributor.googleauthor | 김일찬 | - |
dc.contributor.googleauthor | 이영미 | - |
dc.contributor.googleauthor | 이재승 | - |
dc.relation.code | 2012202143 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | JSLee2 | - |
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