Human Parathyroid Hormone Increases the mRNA Expression of the IGF System and Hematopoietic Growth Factors in Osteoblasts, but Does Not Influence Expression in Mesenchymal Stem Cells
- Title
- Human Parathyroid Hormone Increases the mRNA Expression of the IGF System and Hematopoietic Growth Factors in Osteoblasts, but Does Not Influence Expression in Mesenchymal Stem Cells
- Author
- 이영호
- Keywords
- cord blood; human parathyroid hormone; insulin-like growth factor; mesenchymal stem cell; osteoblast
- Issue Date
- 2012-10
- Publisher
- LIPPINCOTT WILLIAMS & WILKINS, 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA
- Citation
- Journal of Pediatric Hematology/Oncology, Vol.34, No.7 [2012], p491-496
- Abstract
- Osteoblasts, which are derived from pluripotent mesenchymal stem cells (MSCs), play an important role in hematopoiesis. Human parathyroid hormone (hPTH) induces osteoblasts to produce many factors that are essential to hematopoietic stem cells. However, little is known about the impact of hPTH on MSCs to enhance hematopoiesis. We determined the optimal dose of hPTH that was necessary in vitro for increased osteoblast function. In addition, we compared MSC and osteoblast function to explore the role of hPTH in hematopoiesis. The mRNA expression levels of granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin 6, stromal cell-derived factor 1, insulin-like growth factor 1 (IGF-1), IGF-2, insulin-like growth factor-binding protein 1 (IGFBP-1), IGFBP-2, and IGFBP-3 were comparable in osteoblasts and human cord blood-derived MSCs. However, G-CSF, GM-CSF, IGF-2, IGFBP-1, IGFBP-2, and IGFBP-3 expression levels in osteoblasts were markedly increased after treatment with 50 or 100 nM of hPTH. In conclusion, hPTH does not affect the ability of MSCs to differentiate into osteoblasts. In addition, hPTH may enhance hematopoiesis by activating the IGF system (IGF-2, IGFBP-1, IGFBP-2, and IGFBP-3) and hematopoietic growth factors (G-CSF and GM-CSF) in osteoblasts, but not in MSCs.
- URI
- https://journals.lww.com/jpho-online/pages/articleviewer.aspx?year=2012&issue=10000&article=00001&type=abstracthttp://hdl.handle.net/20.500.11754/39581
- ISSN
- 1077-4114
- DOI
- 10.1097/MPH.0b013e318266c0ef
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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