Log-scale dose response of inhibitors on a chip
- Title
- Log-scale dose response of inhibitors on a chip
- Author
- Jong Wook Hong
- Keywords
- MATRIX-METALLOPROTEINASE INHIBITORS; MICROFLUIDIC DEVICE; KINETIC-PARAMETERS; ENZYME ASSAYS; GENERATION; MICROCHIP; GRADIENT; READER; CELLS
- Issue Date
- 2011-08
- Publisher
- AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
- Citation
- ANALYTICAL CHEMISTRY, 권: 83, 호: 16, 페이지: 6148-6153
- Abstract
- We demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirec 0000005E t dilution capability of reagents for the determination of the half inhibitory concentration o 00000AEC r IC(50). The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC(50) of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry.
- URI
- http://pubs.acs.org/toc/ancham/83/16/?http://hdl.handle.net/20.500.11754/37830
- ISSN
- 0003-2700
- DOI
- 10.1021/ac201177g
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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