Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jong Wook Hong | - |
dc.date.accessioned | 2018-02-15T19:31:37Z | - |
dc.date.available | 2018-02-15T19:31:37Z | - |
dc.date.issued | 2011-08 | - |
dc.identifier.citation | ANALYTICAL CHEMISTRY, 권: 83, 호: 16, 페이지: 6148-6153 | en_US |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | http://pubs.acs.org/toc/ancham/83/16/? | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/37830 | - |
dc.description.abstract | We demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirec 0000005E t dilution capability of reagents for the determination of the half inhibitory concentration o 00000AEC r IC(50). The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC(50) of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry. | en_US |
dc.description.sponsorship | J.Y.Y. and S.J. contributed to this work equally. This research was partially supported by a grant from Marine Bioprocess Research Center of the Marine Bio 21 Project funded by the Ministry of Land, Transport and Maritime, Republic of Korea. We also acknowledge partial support from the National Institutes of Health (NIH Grant R01 008392). We thank Jing Dai and Kirn Cramer for their help during the preparation of the manuscript. | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA | en_US |
dc.subject | MATRIX-METALLOPROTEINASE INHIBITORS | en_US |
dc.subject | MICROFLUIDIC DEVICE | en_US |
dc.subject | KINETIC-PARAMETERS | en_US |
dc.subject | ENZYME ASSAYS | en_US |
dc.subject | GENERATION | en_US |
dc.subject | MICROCHIP | en_US |
dc.subject | GRADIENT | en_US |
dc.subject | READER | en_US |
dc.subject | CELLS | en_US |
dc.title | Log-scale dose response of inhibitors on a chip | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1021/ac201177g | - |
dc.relation.page | 6148-6153 | - |
dc.relation.journal | ANALYTICAL CHEMISTRY | - |
dc.contributor.googleauthor | Kim, Se-Kwon | - |
dc.contributor.googleauthor | Jambovane, Sachin | - |
dc.contributor.googleauthor | Yun, Jae Young | - |
dc.contributor.googleauthor | Jambovane, Sachin | - |
dc.contributor.googleauthor | Hong, Jong Wook | - |
dc.contributor.googleauthor | Kim, Se-Kwon | - |
dc.contributor.googleauthor | Cho, Sung-Hak | - |
dc.contributor.googleauthor | Duin, Evert C. | - |
dc.relation.code | 2011200599 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jwh | - |
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