Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이양순 | - |
dc.date.accessioned | 2018-02-07T06:59:50Z | - |
dc.date.available | 2018-02-07T06:59:50Z | - |
dc.date.issued | 2011-07 | - |
dc.identifier.citation | Annals of Laboratory Medicine, 2011, 31(3), P.191-196 | en_US |
dc.identifier.issn | 1598-6535 | - |
dc.identifier.uri | https://synapse.koreamed.org/DOIx.php?id=10.3343/kjlm.2011.31.3.191 | - |
dc.description.abstract | Background: Clostridium difficile infection (CDI) has markedly risen and is associated with hypervirulent ribotype 027 outbreaks in North America and Europe since 2003. The aims of this study were to determine the prevalence of ribotype 027 among C. difficile isolates in Korea, to characterize the ribotype 027 isolates, and to determine the clinical severity of CDI in patients infected with these isolates. Methods: A total of 1,251 isolates of C. difficile recovered from stool specimens of suspected CDI patients at two tertiary-care hospitals and one commercial laboratory between 2002 and 2009. Genes for toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA and cdtB) were detected by PCR. Mutation in the tcdC gene was detected by sequencing after PCR amplification. For molecular genotyping, we performed PCR-ribotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Minimum inhibitory concentrations of moxifloxacin were determined using Etest strips (AB bioMerieux, Sweden). Results: We identified 7 isolates as ribotype 027. These isolates had the same tcdC mutation as the epidemic strain, and 6 of them were resistant to moxifloxacin. The isolates were categorized into 3 different PFGE types and 7 different MLVA types. All the 7 cases had occurred sporadically. Conclusions: C. difficile ribotype 027 is uncommon, but it has emerged in Korea. The spread of this ribotype should be closely monitored in order to avoid an outbreak of CDI in Korea. | en_US |
dc.description.sponsorship | This study was supported by a faculty research grant from Yonsei University College of Medicine (grant 6-2008-0269). We thank Thomas V. Reily (University of Western Australia, Perth, WA, Australia) for providing reference strains and Myungsook Kim (Yonsei University Health System, Seoul, Korea), Kwangwoo Kim (Yonsei University Health System, Seoul, Korea), and Gwanghee Byun (Kyunghee University, Yongin, Korea) for laboratory assistance. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Korean SOC Laboratory Medicine | en_US |
dc.subject | Clostridium difficile | en_US |
dc.subject | PCR-ribotype 027 | en_US |
dc.subject | Pulsed-field gel electrophoresis | en_US |
dc.subject | Multilocus variable-number tandem-repeat analysis | en_US |
dc.title | Emergence of Clostridium difficile Ribotype 027 in Korea | en_US |
dc.type | Article | en_US |
dc.relation.no | 3 | - |
dc.relation.volume | 31 | - |
dc.identifier.doi | 10.3343/kjlm.2011.31.3.191 | - |
dc.relation.page | 191-196 | - |
dc.relation.journal | ANNALS OF LABORATORY MEDICINE | - |
dc.contributor.googleauthor | 김희정 | - |
dc.contributor.googleauthor | 이양순 | - |
dc.contributor.googleauthor | 문희원 | - |
dc.contributor.googleauthor | 임채승 | - |
dc.contributor.googleauthor | 이경원 | - |
dc.contributor.googleauthor | 정윤섭 | - |
dc.contributor.googleauthor | Kim, Heejung | - |
dc.contributor.googleauthor | Lee, Yangsoon | - |
dc.contributor.googleauthor | Moon, Hee-Won | - |
dc.contributor.googleauthor | Lim, Chae Seung | - |
dc.contributor.googleauthor | Lee, Kyungwon | - |
dc.contributor.googleauthor | Chong, Yunsop | - |
dc.relation.code | 2011233374 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | yangsoon | - |
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