Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 강종명 | - |
dc.date.accessioned | 2018-01-30T07:33:12Z | - |
dc.date.available | 2018-01-30T07:33:12Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | AMERICAN JOURNAL OF NEPHROLOGY, v. 33, NO 1, Page. 7-16 | en_US |
dc.identifier.issn | 0250-8095 | - |
dc.identifier.issn | 1421-9670 | - |
dc.identifier.uri | https://www.karger.com/Article/Abstract/322445 | - |
dc.description.abstract | Background/Aims: The increased permeability of chloride in the distal cortical nephron in cyclosporine nephrotoxicity may involve the transcellular pathway mediated by the thiazide-sensitive Na+-Cl? cotransporter and/or the paracellular pathway mediated by the tight junctions (TJs). Methods: Cyclosporine was subcutaneously administered to Sprague-Dawley rats for 6 (7.5 mg/kg body weight) and 2 (25 mg/kg body weight) weeks, and immunoblot analysis and immunohistochemistry were carried out from the kidneys. Electrically tight epithelial Madin-Darby canine kidney (MDCK) I cells were exposed to cyclosporine for 72 h to measure changes in transepithelial electrical resistance (ΔTER). Results: Cyclosporine treatment induced a decrease in Na+-Cl? cotransporter in rat renal cortex. WNK4 protein was increased in both rat kidneys and MDCK I cells. Occludin was also increased in rat kidneys and MDCK I cells exposed to 100 ng/ml cyclosporine. In contrast, cyclosporine treatment induced a decrease in zonula occludens 1 protein abundance and no changes in claudin-1 and claudin-4 in both rat kidneys and MDCK I cells. As a measure of the barrier to small ions, ΔTER of MDCK monolayers was decreased by 100 ng/ml cyclosporine. Conclusion: Renal TJ proteins are affected by cyclosporine treatment. Changes in TJ protein assembly induced by altered expression of WNK4, occludin, and zonula occludens 1 may affect paracellular permeability. | en_US |
dc.description.sponsorship | This study was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund; KRF-2007-313-E00239). | en_US |
dc.language.iso | en | en_US |
dc.publisher | KARGER | en_US |
dc.subject | Cyclosporine nephrotoxicity | en_US |
dc.subject | Paracellular permeability | en_US |
dc.subject | Sodium chloride symporter | en_US |
dc.subject | Tight junction proteins | en_US |
dc.subject | WNK4 protein | en_US |
dc.title | Altered Expression of Tight Junction Proteins in Cyclosporine Nephrotoxicity | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 33 | - |
dc.identifier.doi | 10.1159/000322445 | - |
dc.relation.page | 7-16 | - |
dc.relation.journal | AMERICAN JOURNAL OF NEPHROLOGY | - |
dc.contributor.googleauthor | Lee, Chang Hwa | - |
dc.contributor.googleauthor | Kim, Sua | - |
dc.contributor.googleauthor | Kang, Chong Myung | - |
dc.contributor.googleauthor | Kim, Wan Young | - |
dc.contributor.googleauthor | Kim, Jin | - |
dc.contributor.googleauthor | Kim, Gheun-Ho | - |
dc.relation.code | 2011200496 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | kangjm | - |
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