Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김태환 | - |
dc.date.accessioned | 2017-10-17T01:44:09Z | - |
dc.date.available | 2017-10-17T01:44:09Z | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | ARTHRITIS RESEARCH & THERAPY, v. 17, NO 350, Page. 35-43 | en_US |
dc.identifier.issn | 1478-6354 | - |
dc.identifier.issn | 1478-6362 | - |
dc.identifier.uri | https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-015-0870-4 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/30053 | - |
dc.description.abstract | Backgound: The formation of bony spurs and ankylosis is a key pathognomic feature in ankylosing spondylitis (AS) and results in functional impairment. The aim of this study was to investigate the role of IL-32 gamma in osteoblast (OB) differentiation and its association with the pathogenesis of AS. Methods: The concentration and expression of IL-32 gamma were evaluated in synovial fluid and tissue from patients with AS, rheumatoid arthritis (RA) and osteoarthritis (OA), using enzyme-linked immunosorbent assay and immunohistochemistry. To establish whether IL-32 gamma affects OB differentiation, we used calvarial cells of IL-32 gamma transgenic (TG) mice or wild-type (WT) mice. To elucidate the mechanism of osteoblastogenesis, levels of regulators were assayed in IL-32 gamma TG mice and in primary OBs after IL-32 gamma stimulation. Results: The IL-32 gamma levels were higher in the synovial fluid of AS patients compared with RA or OA patients and the expression of IL-32 was higher in AS synovia than in RA or OA synovia. Additional IL-32 gamma stimulation in precursor cells enhanced OB differentiation potentially and IL-32 gamma TG mice showed higher rates of OB differentiation than WT mice. IL-32 gamma reduced the expression of DKK-1, a negative regulator, in both WT precursor cells and human OBs and the constitutive expression of DKK-1 was suppressed in calvarial cells from IL-32 gamma TG mice. Conclusions: The elevated level of IL-32 gamma in AS joint could enhance OB differentiation via DKK-1 suppression. Therefore, IL-32 gamma might be a putative molecular target to prevent the abnormal bone formation in AS. | en_US |
dc.description.sponsorship | This research was supported by the National Research Foundation of Korea (NRF-2013R1A1A1009271) and the Asan Institute for Life Science (2014-463), with funding from both sources awarded to YGK. EJC received a grant from the Korean Health Technology R&D Project, Ministry of Health & Welfare of Korea (HI11C05070200). EJuL was supported by the National Research Foundation of Korea (NRF-2013R1A1A2059597). | en_US |
dc.language.iso | en | en_US |
dc.publisher | BIOMED CENTRAL LTD | en_US |
dc.subject | Interleukin-32 | en_US |
dc.subject | Osteoblast differentiation | en_US |
dc.subject | Ankylosing spondylitis | en_US |
dc.title | High level of interleukin-32 gamma in the joint of ankylosing spondylitis is associated with osteoblast differentiation | en_US |
dc.type | Article | en_US |
dc.relation.no | 350 | - |
dc.relation.volume | 17 | - |
dc.identifier.doi | 10.1186/s13075-015-0870-4 | - |
dc.relation.page | 35-43 | - |
dc.relation.journal | ARTHRITIS RESEARCH & THERAPY | - |
dc.contributor.googleauthor | Lee, Eun-Ju | - |
dc.contributor.googleauthor | Lee, Eun-Jin | - |
dc.contributor.googleauthor | Chung, Yeon-Ho | - |
dc.contributor.googleauthor | Song, Da-Hyun | - |
dc.contributor.googleauthor | Hong, Seokchan | - |
dc.contributor.googleauthor | Lee, Chang-Keun | - |
dc.contributor.googleauthor | Yoo, Bin | - |
dc.contributor.googleauthor | Kim, Tae-Hwan | - |
dc.contributor.googleauthor | Park, Ye-Soo | - |
dc.contributor.googleauthor | Kim, Soo-Hyun | - |
dc.relation.code | 2015002075 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | thkim | - |
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