FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons

Title
FGF8 is Essential for Functionality of Induced Neural Precursor Cell-derived Dopaminergic Neurons
Authors
임미선
Keywords
Direct conversion; FGF8; Dopamine neuron; iNPC
Issue Date
2015-08
Publisher
Korean Society for Stem Cell Research
Citation
International Journal of Stem Cells, v. 8, NO 2, Page. 228-234
Abstract
Induced neural precursor cells (iNPCs) are one source of transplantable dopaminergic neurons used in cell therapy for Parkinson’s disease. In the present study, we demonstrate that iNPCs can be generated by transducing Brn2, Ascl1, Myt1L and Bcl-xL in a culture supplemented with several mitogens and subsequently can be differentiated to dopaminergic neurons (DA). However, studies have shown that iDA and/or iNPC-derived DA neurons using various conversion protocols have low efficiency. Here, we show that early exposure of FGF8 to fibroblasts efficiently improves differentiation of DA neurons. So our study demonstrates that FGF8 is a critical factor for generation of iNPC-derived DA neurons.
URI
http://www.ijstemcell.com/journal/view.html?uid=163&sort=&scale=&key=year&keyword=&s_v=8&s_n=2&pn=vol&year=2015&vmd=Fullhttp://hdl.handle.net/20.500.11754/26923
ISSN
2005-3606; 2005-5447
DOI
http://dx.doi.org/10.15283/ijsc.2015.8.2.228
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RESEARCH INSTITUTE[S](부설연구소) > ETC
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