Surface-enhanced Raman Scattering-based Competitive Immunoassay of Poly-gamma-D-Glutamic Acid for Highly Sensitive Detection of Anthrax Biomarker

Title
Surface-enhanced Raman Scattering-based Competitive Immunoassay of Poly-gamma-D-Glutamic Acid for Highly Sensitive Detection of Anthrax Biomarker
Authors
주재범
Keywords
Surface-enhanced Raman scattering; Bacillus anthracis; Poly-gamma-D-glutamic acid; Competitive immunoassay; Magnetic bead
Issue Date
2015-03
Publisher
KOREAN CHEMICAL SOC
Citation
BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 36, Page. 822-826
Abstract
Anthrax is a highly acute disease caused by the bacterium Bacillus anthracis. In this article, we report a highly sensitive detection technique for pathogenic B. anthracis, based on the use of a surface-enhanced Raman scattering (SERS)-active magnetic sensor. Gold nanoparticles (GNPs) and magnetic beads were used as SERS nanotags and supporting substrates, respectively. Here, an SERS-based competitive immunoassay platform is described for the quantitative evaluation of the anthrax marker. Poly-gamma-D-glutamic acid (PGA) capsules were used as a target biomarker for the highly sensitive detection of B. anthracis becausePGAis known to be closely associated with the pathogenesis of B. anthracis infection. The SERS-based competitive assay reveals an extraordinarily high sensitivity for PGA with a limit of detection of similar to 1.0 ng/mL. This work provides a conceptually new immunoassay prototype for fast and sensitive detection of PGA markers.
URI
http://onlinelibrary.wiley.com/doi/http://onlinelibrary.wiley.com/doi/10.1002/bkcs.10154/fullhttp://hdl.handle.net/20.500.11754/23219
ISSN
0253-2964; 1229-5949
DOI
http://dx.doi.org/10.1002/bkcs.10154
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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