Nanomechanical measurement of astrocyte stiffness correlated with cytoskeletal maturation.

Title
Nanomechanical measurement of astrocyte stiffness correlated with cytoskeletal maturation.
Author
오재응
Keywords
ex vivo culture; astrocyte; atomic force microscopy; cell stiffness; cytoskeletal structure
Issue Date
2015-01
Publisher
WILEY-BLACKWELL
Citation
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Volume 103, Issue 1, Page. 365-370
Abstract
Astrocytes are known to serve as scaffolding cells that shape the brain. The physical properties of astrocytes, such as stiffness, are important for their scaffolding function. These properties may be altered in certain pathological conditions, such as in brain cancer. However, actual stiffness of astrocytes is not yet well understood. Here, we report that the astrocyte stiffness is positively correlated with the density of cytoskeletal proteins, such as actin filaments, microtubules, and intermediate filaments. The value of the stiffness of astrocytes as measured by atomic force microscopy (AFM) increases 38-fold in five-week-old rats compared to postnatal-day zero pups. Using multicolor confocal microscopy, we found that the complexity of cytoskeletal proteins, such as actin filaments, microtubules, and intermediate filaments, increase as the animal gets older. Our findings indicate that the change of stiffness positively correlates with the maturation of cytoskeletal proteins, and suggest that AFM can be useful as an analytical and diagnostic tool for neuroscience. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 365-370, 2015.
URI
http://hdl.handle.net/20.500.11754/21489http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35174/full
ISSN
1549-3296
DOI
10.1002/jbm.a.35174
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > MECHANICAL ENGINEERING(기계공학부) > Articles
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