In vitro expansion of human adipose-derived stem cells in a spinner culture system using human extracellular matrix powders
- Title
- In vitro expansion of human adipose-derived stem cells in a spinner culture system using human extracellular matrix powders
- Author
- 조용우
- Keywords
- Adipose-derived stem cell; hECM powders; 3-D culture system; Large expansion; Autologous tissue engineering; Human
- Issue Date
- 2011-09
- Publisher
- SPRINGER
- Citation
- CELL AND TISSUE RESEARCH, v. 345, NO. 3, Page. 415-423
- Abstract
- Stem cell therapy requires large numbers of stem cells to replace damaged tissues, but only limited numbers of stem cells can be harvested from a single patient. To obtain large quantities of stem cells with differentiation potential, we explored a spinner culture system using human extracellular matrix (hECM) powders. The hECM was extracted from adipose tissue and fabricated into powders. Human adipose-derived stem cells (hASCs) were isolated, seeded on hECM powders, and cultivated in a spinner flask. The 3-D culture system, using hECM powders, was highly effective for promoting cell proliferation. The number of hASCs in the 3-D culture system significantly increased for 10 days, resulting in an approximately 10-fold expansion, whereas a traditional 2-D culture system showed just a 2.8-fold expansion. Surface markers, transcriptional factors, and differentiation potential of hASCs were assayed to identify the characteristics of proliferated cells in 3-D culture system. The hASCs expressed the pluripotency markers, Oct-4 and Sox-2 during 3-D culture and retained their capacity to differentiate into adipogenic, osteogenic, and chondrogenic lineages. These findings demonstrate that the 3-D culture systems using hECM powders provide an efficient in vitro environment for stem cell proliferation, and could act as stem cell delivery carriers for autologous tissue engineering and cell therapy.
- URI
- https://link.springer.com/article/10.1007%2Fs00441-011-1223-5https://repository.hanyang.ac.kr/handle/20.500.11754/183762
- ISSN
- 0302-766X;1432-0878
- DOI
- 10.1007/s00441-011-1223-5
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MATERIALS SCIENCE AND CHEMICAL ENGINEERING(재료화학공학과) > Articles
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