시료주입시 기포발생이 억제된 반응조 형태의 중합효소연쇄반응용 PDMS/유리 바이오칩
- Title
- 시료주입시 기포발생이 억제된 반응조 형태의 중합효소연쇄반응용 PDMS/유리 바이오칩
- Other Titles
- PDMS/Glass Serpentine Microchannel Chip for PCR with Bubble Suppression in Sample Injection
- Author
- 황승용
- Keywords
- Polymerase Chain Reaction(중합효소연쇄반응); Serpentine Microchannel Reactor(사선 마이크로채널 반응조); PDMS/Glass DNA Chip (PDMS/유리 유전자칩)
- Issue Date
- 2006-10
- Publisher
- 대한기계학회
- Citation
- 대한기계학회논문집 A, v. 30, NO. 10, Page. 1261-1268
- Abstract
- This paper reports low-cost microreactor (10㎕) biochip for the DNA PCR (polymerase chain reaction). The microbiochip (20mm × 28mm) is a hybrid type which is composed of PDMS (polydimethylsiloxane) layer with serpentine micochannel (360㎛x100㎛) chamber and glass substrate integrated with microheater and thermal microsensor. Undesirable bubble is usually created during sample loading to PMDS-based microchip because of hydrophobic chip surface. Created bubbles interrupt stable biochemical reaction. We designed improved microreactor chamber using microfluidic simulation. The designed reactor has a corner-rounded serpentine channel architecture, which enables stable injection into hydrophobic surface using micropipette only. Reactor temperature needed to PCR reaction is controlled within 0.5℃ by PID controller of LabVIEW software. It is experimentally confirmed that SRY gene PCR by the fabricated microreactor chip is performed for less than 54 min.
- URI
- https://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE00762836https://repository.hanyang.ac.kr/handle/20.500.11754/181692
- ISSN
- 1226-4873
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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