Development of microbiochip for detection of metalloproteinase 7 using fluorescence resonance energy transfer
- Title
- Development of microbiochip for detection of metalloproteinase 7 using fluorescence resonance energy transfer
- Author
- 황승용
- Keywords
- Microbiochip; Matrix Metalloproteinase 7; Fluorescence Resonance Energy Transfer (FRET); Quantum Dot (QD); Bead-based assay; Enzyme assay
- Issue Date
- 2013-06
- Publisher
- 한국바이오칩학회
- Citation
- BioChip Journal, v. 7, NO. 2, Page. 164-172
- Abstract
- A protease is any enzyme that catalyzes the hydrolysis of proteins into smaller peptide fragments and amino acids, a process known as proteolysis. They are involved in a multitude of normal biological processes as well as in diseases, including cancer, stroke and infections. Here we present a microfluidic-based assay system to detect proteolytic activity using fluorescence resonance energy transfer (FRET) by quantum dot (QD)-peptide conjugates immobilized on microbeads. As an energy donor, QD was immobilized on the microbead surface by the avidin-biotin interaction. As an energy acceptor, the fluorophore-labeled peptide was then associated with QD, thus quenching the photoluminescence (PL) of the QD. The functionalized microbeads were introduced into the microbiochip and captured by a micropillar in the reaction chamber. In the presence of matrix metalloprotease-7 (MMP-7) as a model protease, the PL of QD quenched by fluorophore was recovered due to the proteolytic activity of MMP-7 in the fabricated microbiochip. Moreover, the FRET efficiency induced by MMP-7 was linearly dependent on the logarithmic concentration of MMP-7. This technology is not limited to sensing MMP-7, but could be used to monitor other protease activities (Schematic diagram).
- URI
- https://link.springer.com/article/10.1007%2Fs13206-013-7210-zhttps://repository.hanyang.ac.kr/handle/20.500.11754/181617
- ISSN
- 1976-0280;2092-7843
- DOI
- 10.1007/s13206-013-7210-z
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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