Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전대원 | - |
dc.date.accessioned | 2022-10-25T00:31:35Z | - |
dc.date.available | 2022-10-25T00:31:35Z | - |
dc.date.issued | 2021-02 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF STEM CELLS, v. 14, no. 2, page. 221-228 | en_US |
dc.identifier.issn | 2005-3606; 2005-5447 | en_US |
dc.identifier.uri | https://www.ijstemcell.com/journal/view.html?doi=10.15283/ijsc20096 | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/175756 | - |
dc.description.abstract | Background and Objectives: Hypoxia is frequently used to enhance stem cell function. However, the optimal level of hypoxia for growth and function of human embryonic stem cell-derived mesenchymal stem cells (hES-MSCs) is yet to be determined. The purpose of this study was to find the optimal level of hypoxia for hES-MSCs and characteristics of hES-MSCs cultured under these optimal hypoxic conditions. Methods and Results: Cell viability and changes in the morphology of hES-MSCs were determined through cell proliferation and CCK-8 assay. The hES-MSCs were preconditioned under various hypoxic conditions (0.5∼5% O2 and 24∼72 h). The expression of cytokines in each culture condition was compared using cytokine array analysis. The morphology of hES-MSCs did not change under various hypoxic culture conditions. hES-MSCs viability after 48 h incubation in 2% O2 condition was higher than that in normoxic condition. HIF1α expression was increased up to six folds after 48 h of hypoxic preconditioning. HIF1α expression in hES-MSCs peaked after 48 h of incubation in 1% O2 condition. The expressions of PDGF-BB, IGFBP-6, VEGF-A, and angiogenin were increased after hES-MSCs were incubated for 48 h in 2% O2 condition. Conclusions: The hES-MSCs viability and expressions of PDGF-BB, IGFBP-6, VEGF-A, and angiogenin increased after 48 h incubation in 2% O2 condition. | en_US |
dc.description.sponsorship | This study was funded by the National Research Foundation of Korea (NRF-2017R1C1B5074215) and Daewoong Pharmaceuticals Co., Ltd. | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN SOC STEM CELL RESEARCH | en_US |
dc.subject | Hypoxia; Stem cell; Function; VEGF | en_US |
dc.title | Optimal Hypoxic Preconditioning of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells (hES-MSCs) and Their Characteristics | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.15283/ijsc20096 | en_US |
dc.relation.page | 1-8 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF STEM CELLS | - |
dc.contributor.googleauthor | Lee, Seung Min | - |
dc.contributor.googleauthor | Jun, Dae Won | - |
dc.contributor.googleauthor | Kang, Hyeon Tae | - |
dc.contributor.googleauthor | Oh, Ju Hee | - |
dc.contributor.googleauthor | Saeed, Waqar Khalid | - |
dc.contributor.googleauthor | Ahn, Sang Bong | - |
dc.relation.code | 2021006667 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | noshin | - |
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