Ginsenoside 20(S)-protopanaxadiol induces cell death in human endometrial cancer cells via apoptosis
- Title
- Ginsenoside 20(S)-protopanaxadiol induces cell death in human endometrial cancer cells via apoptosis
- Author
- 이창호
- Keywords
- endometrial cancer; 20(S)-PPD; apoptosis; xenograft; athymic mice
- Issue Date
- 2021-01
- Publisher
- KOREAN SOC GINSENG
- Citation
- JOURNAL OF GINSENG RESEARCH, v. 45, no. 1, page. 126-133
- Abstract
- Background: 20(S)-protopanaxadiol (20(S)-PPD), one of the aglycone derivatives of major ginsenosides, has been shown to have an anticancer activity toward a variety of cancers. This study was initiated with an attempt to evaluate its anti-cancer activity toward human endometrial cancer by cell and xenograft mouse models.
Methods: Human endometrial cancer (HEC)- 1A cells were incubated with different 20( S)-PPD concentrations. 20(S)-PPD cytotoxicity was evaluated using MTT assay. Apoptosis was detected using the annexin V binding assay and cell cycle analysis. Cleaved poly (ADP-ribose) polymerase (PARP) and activated caspase-9 were assessed using western blotting. HEC-1A cell tumor xenografts in athymic mice were generated by inoculating HEC-1A cells into the flank of BALB/c female mice and explored to validate 20(S)-PPD anti-endometrial cancer toxicity.
Results: 20(S)-PPD inhibited HEC-1A cell proliferation in a dose-dependent manner with an IC50 value of 3.5 mM at 24 h. HEC-1A cells morphologically changed after 20(S)-PPD treatment, bearing resemblance to Taxol-treated cells. Annexin V-positive cell percentages were 0%, 10.8%, and 58.1% in HEC-1A cells when treated with 0, 2.5, and 5 mM of 20(S)-PPD, respectively, for 24 h. 20(S)-PPD subcutaneously injected into the HEC-1A cell xenograft-bearing mice three times a week for 17 days manifested tumor growth inhibition by as much as 18% at a dose of 80 mg/kg, which sharply contrasted to controls that showed an approximately 2.4-fold tumor volume increase. These events paralleled caspase-9 activation and PARP cleavage.
Conclusion: 20(S)-PPD inhibits endometrial cancer cell proliferation by inducing cell death via a caspasemediated apoptosis pathway. Therefore, the 20(S)-PPD-like ginsenosides are endowed with ample structural information that could be utilized to develop other ginsenoside-based anticancer agents. (C) 2020 The Korean Society of Ginseng. Publishing services by Elsevier B.V.
- URI
- https://www.sciencedirect.com/science/article/pii/S1226845320300579?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/175286
- ISSN
- 1226-8453; 2093-4947
- DOI
- 10.1016/j.jgr.2020.02.002
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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