Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 전병훈 | - |
dc.date.accessioned | 2022-10-12T01:06:07Z | - |
dc.date.available | 2022-10-12T01:06:07Z | - |
dc.date.issued | 2021-01 | - |
dc.identifier.citation | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 208, article no. 111742, Page. 1-12 | en_US |
dc.identifier.issn | 0147-6513 ; 1090-2414 | en_US |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0147651320315785?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/175228 | - |
dc.description.abstract | The indiscriminate disposal of olsalazine in the environment poses a threat to human health and natural ecosystems because of its cytotoxic and genotoxic nature. In the present study, degradation efficiency of olsalazine by the marine-derived fungus, Aspergillus aculeatus (MT492456) was investigated. Optimization of physicochemical parameters (pH. Temperature, Dry weight) and redox mediators {(2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), p-Coumaric acid and 1-hydroxybenzotriazole (HOBT)} was achieved with Response Surface Methodology (RSM)-Box-Behnken Design (BBD) resulting in 89.43% removal of olsalazine on 7th day. The second-order polynomial regression model was found to be statistically significant, adequate and fit with p < 0.0001, F value=41.87 and correlation coefficient (R2=0.9826). Biotransformation was enhanced in the redox mediator-laccase systems resulting in 99.5% degradation of olsalazine. The efficiency of ABTS in the removal of olsalazine was more pronounced than HOBT and p-Coumaric acid in the laccase-mediator system. This is attributed to the potent nature of the electron transfer mechanism deployed during oxidation of olsalazine. The pseudo-second-order kinetics revealed that the average half-life () and removal rates () increases with increasing concentrations of olsalazine. Michaelis-Menten kinetics affirmed the interaction between laccase and olsalazine under optimized conditions with maximum removal rate, =111.11 hr-1 and half-saturation constant, =1537 mg L-1. At the highest drug concentration (2 mM); 98%, 95% and 93% laccase was remarkably stabilized in the enzyme-drug degradation system by HOBT, ABTS and p-Coumaric acid respectively. This study further revealed that the deactivation of laccase by the redox mediators is adequately compensated with enhanced removal of olsalazine. | en_US |
dc.description.sponsorship | BPO hereby express his profound thanks to the management of Federal University of Agriculture Abeokuta, Ogun State, Nigeria for the administrative and institutional support. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.subject | Olsalazine; Laccase; Redox-mediators; Box-Behnken design (BBD); Polycyclic non-steroidal anti-inflammatory drugs | en_US |
dc.title | Impact of redox-mediators in the degradation of olsalazine by marine-derived fungus, Aspergillus aculeatus strain bpo2: Response surface methodology, laccase stability and kinetics | en_US |
dc.type | Article | en_US |
dc.relation.volume | 208 | - |
dc.identifier.doi | 10.1016/j.ecoenv.2020.111742 | en_US |
dc.relation.page | 1-12 | - |
dc.relation.journal | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | - |
dc.contributor.googleauthor | Bankole, Paul Olusegun | - |
dc.contributor.googleauthor | Semple, Kirk Taylor | - |
dc.contributor.googleauthor | Jeon, Byong-Hun | - |
dc.contributor.googleauthor | Govindwar, Sanjay Prabhu | - |
dc.relation.code | 2021004733 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING | - |
dc.identifier.pid | bhjeon | - |
dc.identifier.orcid | https://orcid.org/0000-0002-5478-765X | - |
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