Performance and limitation estimation of a three-tap gated imaging sensor in wide field time-gated fluorescence lifetime imaging systems
- Title
- Performance and limitation estimation of a three-tap gated imaging sensor in wide field time-gated fluorescence lifetime imaging systems
- Author
- 이지원
- Issue Date
- 2021-08
- Publisher
- OPTICAL SOC AMER
- Citation
- APPLIED OPTICS; AUG 20 2021, 60 24, p7446-p7454, 9p.
- Abstract
- In this paper, a computational performance analysis is presented of a wide-field time-gated fluorescence lifetime
imaging microscope (FLIM) using practically realizable properties of the laser, sample, and a three-tap time-gated
CMOS image sensor. The impact of these component-level properties on the accuracy and the precision of the
measurement results are estimated and discussed based on Monte Carlo simulations. The correlation between
the detector speed and the accuracy of the extracted fluorescence lifetime is studied, and the minimum required
incident photoelectron number of each pixel is estimated for different detector speeds and different fluorescence
lifetime measurements. In addition, the detection limits due to the dark current and the parasitic light sensitivity
of the detector are also investigated. This work gives an overview of the required fluorescence emission condition
as well as the required detector properties for a three-tap time-gated image sensor to achieve good FLIM data in
biological applications.
- URI
- https://opg.optica.org/ao/fulltext.cfm?uri=ao-60-24-7446&id=457336https://repository.hanyang.ac.kr/handle/20.500.11754/172574
- ISSN
- 1559128X; 21553165
- DOI
- 10.1364/AO.428590
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > PHOTONICS AND NANOELECTRONICS(나노광전자학과) > Articles
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