Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조용우 | - |
dc.date.accessioned | 2021-09-09T05:04:30Z | - |
dc.date.available | 2021-09-09T05:04:30Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.citation | BIOTECHNOLOGY JOURNAL, v. 15, no. 12, Article no. 2000079, 6pp | en_US |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.issn | 1860-7314 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/full/10.1002/biot.202000079 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/165036 | - |
dc.description.abstract | The topical delivery of siRNA-based therapies has opened new avenues for the treatment of skin disorders. The use of siRNA as a therapeutic, however, is limited due to its rapid degradation and poor cellular uptake. Furthermore, the top layer of skin, the stratum corneum, is a major barrier to the delivery of topical agents. There is an unmet need for efficient topical formulations for delivering siRNA to the site of action. In this study, 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or lipofectamine is used to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) GAPDH expression is then evaluated at the cellular level. In addition, a dermal transport assay is designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier is significantly better than the delivery of siRNA without it. Thus, the findings identify lipid nanocarriers as excellent candidates for the transdermal delivery of siRNA for gene silencing in the skin and thus for applications in related preclinical models. | en_US |
dc.description.sponsorship | This research was supported by the Chung-Ang University Research Grant in 2019. This study was also supported by the Nano-Material Technology Development Program through the National Research Foundation of Korea (NRF), funded by the Korean government (No. 2017M3A7B8061942). This study was also partially supported and funded by the AMOREPACIFIC R&D Unit. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | WILEY-V C H VERLAG GMBH | en_US |
dc.subject | 1,2-dioleoyl-3-trimethylammonium-propane | en_US |
dc.subject | gene delivery | en_US |
dc.subject | lipofectamine | en_US |
dc.subject | nanocarrier | en_US |
dc.subject | siRNA | en_US |
dc.subject | transdermal | en_US |
dc.title | Study and Evaluation of the Potential of Lipid Nanocarriers for Transdermal Delivery of siRNA | en_US |
dc.type | Article | en_US |
dc.relation.no | 12 | - |
dc.relation.volume | 15 | - |
dc.identifier.doi | 10.1002/biot.202000079 | - |
dc.relation.page | 1-6 | - |
dc.relation.journal | BIOTECHNOLOGY JOURNAL | - |
dc.contributor.googleauthor | Lee, Kyungwoo | - |
dc.contributor.googleauthor | Min, Daejin | - |
dc.contributor.googleauthor | Choi, Yonghyun | - |
dc.contributor.googleauthor | Kim, Jiwon | - |
dc.contributor.googleauthor | Yoon, Semi | - |
dc.contributor.googleauthor | Jang, Jaehee | - |
dc.contributor.googleauthor | Park, Soomin | - |
dc.contributor.googleauthor | Tanaka, Masayoshi | - |
dc.contributor.googleauthor | Cho, Yong Woo | - |
dc.contributor.googleauthor | Koo, Hyung-Jun | - |
dc.relation.code | 2020049454 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING | - |
dc.identifier.pid | ywcho7 | - |
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