Characterization of a novel LQT3 variant with a selective efficacy of mexiletine treatment
- Title
- Characterization of a novel LQT3 variant with a selective efficacy of mexiletine treatment
- Author
- 박종은
- Keywords
- LONG-QT SYNDROME; CHANNEL MUTATIONS; SCN5A; GENE; INACTIVATION; ARRHYTHMIA; GENOTYPE; THERAPY; EVENTS
- Issue Date
- 2019-09
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v. 9, article no. 12997
- Abstract
- Pathogenic variants in the human SCINISA gene encoding the a-subunit of the principle Na+ channel (Nav1.5) are associated with long QT syndrome (LQTS) 3. LQT3 patients display variable responses to Na+ channel blockers demanding for the development of variant-specific therapeutic strategies. Here we performed a combined electrophysiological analysis with in silico simulation of variant channel to elucidate mechanisms of therapeutic responsiveness. We identified a novel SCINISA variant (A1656D) in a LQTS patient with a distinct response to mexiletine resulting in suppression of non-sustained ventricular tachycardia and manifestation of premature atrial contraction. Patch clamp analysis revealed that A1656D variant exerted gain-of-function effects including hyperpolarizing shift of the voltage-dependence of activation, depolarizing shift in the voltage-dependence of inactivation, and slowing of fast inactivation. Among ranolazine, flecainide, and mexiletine, only mexiletine restored inactivation kinetics of A1656D currents. In silico simulation to assess the effect of A1656D variant on ventricular cardiac cell excitation predicted a prolonged action potential which is consistent with the prolonged QT and non-sustained ventricular tachycardia of the patient. It also predicted that only mexiletine suppressed the prolonged action potential of human ventricular myocytes expressing A1656D. These data elucidate the underlying mechanism of the distinct response to mexiletine in this patient.
- URI
- https://www.nature.com/articles/s41598-019-49450-0https://repository.hanyang.ac.kr/handle/20.500.11754/161905
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-019-49450-0
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML