Leukotactin-1-induced ERK activation is mediated via Gi/Go protein/PLC/PKCδ/Ras cascades in HOS cells.
- Title
- Leukotactin-1-induced ERK activation is mediated via Gi/Go protein/PLC/PKCδ/Ras cascades in HOS cells.
- Author
- 이영한
- Keywords
- Leukotactin-1; CCR1; Signal transduction; Chemokine; ERK; PLC; PKC
- Issue Date
- 2003-06
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Citation
- LIFE SCIENCES, v. 73, issue. 4, page. 447-459
- Abstract
- Recently cloned leukotactin-1 (Lkn-1) that belongs to CC chemokine family has not been characterized. To understand the intracellular events following Lkn-1 binding to CCR1, we investigated the activities of signaling molecules in response to Lkn-1 in human osteogenic sarcoma cells expressing CCR1. Lkn-1-stimulated cells showed elevated phosphorylation of extracellular signal-related kinases (ERK1/2) with a distinct time course. ERK activation was peaked in 30 min and 12 h showing biphasic activation of ERK. Pertussis toxin, an inhibitor of Gi/Go protein, and phospholipase C (PLC) inhibitor blocked Lkn-1-induced activation of ERK. Protein kinase Cδ (PKCδ) specific inhibitor rottlerin inhibited ERK activation in Lkn-1-stimulated cells. The activities of PLC and PKCδ were also enhanced by Lkn-1 stimulation. Dominant negative Ras inhibited activation of ERK. Immediate early response genes such as c-fos and c-myc were induced by Lkn-1 stimulation. Lkn-1 affected the cell cycle progression by cyclin D3 induction. These results suggest that Lkn-1 activates the ERK pathway by transducing the signal through Gi/Go protein, PLC, PKCδ and Ras, and it may play a role for cell proliferation, differentiation, and regulation of gene expression for other cellular processes.
- URI
- https://www.sciencedirect.com/science/article/pii/S0024320503003126https://repository.hanyang.ac.kr/handle/20.500.11754/155932
- ISSN
- 0024-3205
- DOI
- 10.1016/S0024-3205(03)00312-6
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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