BULLETIN OF THE KOREAN CHEMICAL SOCIETY(대한화학회), v.25, No.12, Page.1845-1849
Abstract
Apolipoprotein B-100 (Apo-B100) is a major protein component for low density lipoproteins (LDL). A number
of mimetic peptides of Apo-B100 were screened from the phase-displayed random peptide library by utilizing
monoclonal antibody (B9). Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF (pB1).
From the BLAST search, the mimetic peptide pB1 had 40% homology with apo B-100. As a result of the
structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based
distance geometry (DG)/molecular dynamic (MD) computations, DG structure had low penalty value of
0.3-0.6 Å2 and the total RMSD was 0.5-1.5 Å. Moreover, pB1 structure included a weak 310-helix from Ile7 to
Trp13.