CRISPR/Cas9-targeted mutagenesis of F3′H, DFR and LDOX, genes related to anthocyanin biosynthesis in black rice (Oryza sativa L.)
- Title
- CRISPR/Cas9-targeted mutagenesis of F3′H, DFR and LDOX, genes related to anthocyanin biosynthesis in black rice (Oryza sativa L.)
- Author
- 배상수
- Keywords
- Black rice; Anthocyanin; OsLDOX; OsF3′H; OsDFR; CRISPR/Cas9; Gene editing
- Issue Date
- 2019-10
- Publisher
- SPRINGER
- Citation
- PLANT BIOTECHNOLOGY REPORTS, v. 13, no. 5, Page. 521-531
- Abstract
- Altering a trait by CRISPR-Cas9-targeted mutagenesis offers great advantages in identifying gene function and crop improvement. In the present study, three genes (OsF3 ' H, OsDFR and OsLDOX) in the anthocyanin biosynthesis pathway were successfully edited on the Heugseonchal or Sinmyungheugchal variety using the CRISPR/Cas9 system. As a result, the ratio of the edited plants in the transformed early generation was 56.7%. These edited mutant lines were observed with the changes of seed color and anthocyanin content. All mutations were stably inherited to the T-2 progeny. In addition, we could select edited homozygous mutant lines lacking the T-DNA already in the first offspring generation. Also the insertion of vector backbone sequences in f3 ' h-9, dfr-4 and ldox-16 lines was not detected in the whole genome resequencing. These results demonstrated that the CRISPR/Cas9 system can induce clearly gene-specific mutations with a high efficiency in rice and plants selected from these mutants cannot be distinguished from non-GMO plants even under strict GMO regulation.
- URI
- https://link.springer.com/article/10.1007%2Fs11816-019-00579-4https://repository.hanyang.ac.kr/handle/20.500.11754/154273
- ISSN
- 1863-5466; 1863-5474
- DOI
- 10.1007/s11816-019-00579-4
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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