Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 하지혜 | - |
dc.date.accessioned | 2020-09-03T07:49:53Z | - |
dc.date.available | 2020-09-03T07:49:53Z | - |
dc.date.issued | 2019-11 | - |
dc.identifier.citation | CLINICA CHIMICA ACTA, v. 498, Page. 11-15 | en_US |
dc.identifier.issn | 0009-8981 | - |
dc.identifier.issn | 1873-3492 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0009898119319904?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/153543 | - |
dc.description.abstract | Background: The detection of hepatitis C virus antibody (anti-HCV) is known to have high false-positive rates. Using signal-to-cutoff (S/Co) ratios in reflex supplemental testing, however, could reduce false-positive rates. Here, we analyzed the 2-year data of an anti-HCV assay to understand the significance of the S/Co ratio and make a new algorithm by confirming with a second anti-HCV assay. Methods: We reviewed 32,573 samples of the Architect assay (Abbott Diagnostics) from a tertiary hospital. Retests with the Elecsys (Roche Diagnostics) and Vitros (Ortho Clinical Diagnostics) assays were performed in 346 anti-HCV-positive samples. HCV RNA PCR and recombinant immunoblot assay (RIBA) were performed in 147 and 11 anti-HCV-positive samples, respectively. Results: Among 32,573 samples, 446 (1.37%) yielded positive results and 32,127 (98.6%) yielded negative results. Concordance rates in low S/Co samples (0.9-10.0) were 35.2%, 43.8%, and 81.9% for the Architect-Elecsys, Architect-Vitros, and Elecsys-Vitros comparisons, respectively. Correlation coefficients of S/Co ratios were as follows: Architect-Elecsys, 0.20; Architect-Vitros, 0.42; and Elecsys-Vitros, 0.46. In logistic regression, the S/Co value for predicting positivity with 95% probability was 3.13, while that for predicting 50% probability was 8.85. S/Co ratios of 1.70-3.34 showed one reactive antigen out of five antigens, and S/Co ratios of 13.54-17.72 showed three to five positive reactions out of five antigens used in the RIBA. Conclusions: Supplementary testing of anti-HCV screening results is necessary to distinguish between true positivity and biological false positivity for anti-HCV. In this study, we presented an algorithm of supplementary testing by a retest with a second reagent, which could be useful in clinical laboratories. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Hepatitis C virus | en_US |
dc.subject | Anti-HCV assays | en_US |
dc.subject | Signal-to-cutoff ratio | en_US |
dc.subject | Immunoassay | en_US |
dc.title | Signal-to-cutoff ratios of current anti-HCV assays and a suggestion of new algorithm of supplementary testing | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.cca.2019.08.002 | - |
dc.relation.journal | CLINICA CHIMICA ACTA | - |
dc.contributor.googleauthor | Ha, Jihye | - |
dc.contributor.googleauthor | Park, Younhee | - |
dc.contributor.googleauthor | Kim, Hyon-Suk | - |
dc.relation.code | 2019003594 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | somatotropin | - |
dc.identifier.orcid | https://orcid.org/0000-0002-0548-0416 | - |
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