감초 추출물 함유 나노캡슐의 특성 및 항산화 활성

Abstract

The principle objective of this study was to develop an effective Glycyrrhiza uralensis extract– loaded nanoparticle for improving oxidative stability. For this purpose, we first optimized the extraction conditions for the maximal antioxidant activity of G. uralensis extract. Then we investigated the effect of various preparation conditions of G. uralensis extract-loaded chitosan nanoparticles on the particle characteristics. Finally, we evaluated the antioxidant activity of free G. uralensis extracts and nanoencapsulated G. uralensis extract. Response surface methodology was used to optimize extraction conditions, including ethanol concentration (X1) and extraction time (X2), for the maximal antioxidant activity of G. uralensis extract. The optimized values of X1 and X2 were found to be 83.01% and 32.6 min, respectively. Experimental results obtained for the optimum extraction conditions agreed favorably with the predicted results, indicating the usefulness of prediction models for antioxidant activity. This potent antioxidant activity had good correlation with total flavonoid content. G. uralensis extract-loaded chitosan nanoparticles prepared by ionic gelation were characterized by particle size, zeta potential, entrapment efficiency, and loading efficiency. The optimum concentration of G. uralensis extract for maximum entrapment efficiency and loading efficiency was found to be 0.8 mg/mL and 2.0 mg/mL, respectively. Both free G. uralensis extract and G. uralensis extract-loaded chitosan nanoparticles showed concentration-dependent antioxidant activity. However, the lipid peroxidation inhibitory activity of G. uralensis extract was effectively enhanced when it was entrapped within chitosan nanoparticles. In conclusion, chitosan nanoparticle encapsulation is therefore a potentially valuable technique for improving the antioxidant activity of G. uralensis extract.