인간 지방유래 줄기세포 엑소좀: 분리, 특성분석 및 기능성 화장품 소재로의 응용

Abstract

Stem cell-derived exosomes contain bioactive substances such as proteins, messenger RNAs, and microRNAs and play important role in tissue regeneration via the transfer of specific bioactive substances. Herein, we hypothesized that exosomes secreted by adipose-derived stem cells (HASCs) have a positive role in promotion of collagen remodeling in UVB-induced skin damage. Exosomes (P-EXOs) were isolated from proliferating HASCs through multi-filtration system (MFS) based on tangential flow filtration (TFF) and characterized by different methodologies. The P-EXOs contained various microRNAs and proteins related to skin development and extracellular matrix (ECM) synthesis. We found that P-EXOs significantly promoted proliferation, migration and collagen synthesis of human fibroblasts, with increased gene expression of tissue inhibitor of megalloproteinase-1/-3 (TIMP-1/-3) and collagen type I alpha 1 (COL1A1). Our findings suggest that P-EXOs act as a biological cue stimulating dermal fibroblasts and provide a new approach for functional cosmetics in skin rejuvenation.